Leadgene Leadgene
Swine FGF-2, His Tag, E. coli
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  • Specifications
    • Species of Origin


      Expression system

      Escherichia coli

    • Affinity Tag

      His Tag (N-term)


      Lyophilized from a 0.2 µm filtered solution of PBS containing 0.01% sarkosyl, pH 7.4.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 18.1 kDa.
      The protein migrates as 17 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to induce proliferation in 3T3 cells. The ED₅₀ for this effect is <2 ng/mL.

      Endotoxin level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form


  • Background
    • Background

      Basic fibroblast Growth Factors (FGF-2, bFGF), a pleiotropic cytokine, plays multiple roles in different cells and tissues. FGF-2 can stimulate smooth muscle cell growth, wound healing, and tissue repair. In addition, FGF-2 has been shown to regulate the generation of neurons and astrocytes from progenitor cells. FGF-2 are also involved in a variety of biological processes, including embryonic development, morphogenesis, tissue repair, tumor growth, and invasion. As a multifunctional cytokine, FGF-2 is first isolated from the pituitary. Later, it was identified from various cell types including cardiac myocytes, cardiac fibroblasts, endothelial cells, and smooth muscle cells.


      Fibroblast Growth Factors

    • Uniprot ID

      # NP_001392443

      Sequence Note


  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.


      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 2 weeks under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant swine FGF-2.

    SDS-PAGE analysis of recombinant swine FGF-2.

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  • Datasheet & Documents

Disclaimer:For Research Use or Further Manufacturing Only.

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