-
Species of Origin
Swine
Expression system
Escherichia coli
-
Affinity Tag
His Tag (N-term)
Buffer
Lyophilized from a 0.2 µm filtered solution of PBS containing 0.01% sarkosyl, pH 7.4.
-
Purity
>98% as determined by SDS-PAGE analysis.
Molecular weight
The protein has a calculated MW of 18.1 kDa.
The protein migrates as 17 kDa under reducing condition (SDS-PAGE analysis). -
Activity
Measure by its ability to induce proliferation in 3T3 cells. The ED₅₀ for this effect is <2 ng/mL.
Endotoxin level
<0.1 EU per 1 μg of the protein by the LAL method.
-
Form
Lyophilized
-
Specifications
-
Background
-
Background
Basic fibroblast Growth Factors (FGF-2, bFGF), a pleiotropic cytokine, plays multiple roles in different cells and tissues. FGF-2 can stimulate smooth muscle cell growth, wound healing, and tissue repair. In addition, FGF-2 has been shown to regulate the generation of neurons and astrocytes from progenitor cells. FGF-2 are also involved in a variety of biological processes, including embryonic development, morphogenesis, tissue repair, tumor growth, and invasion. As a multifunctional cytokine, FGF-2 is first isolated from the pituitary. Later, it was identified from various cell types including cardiac myocytes, cardiac fibroblasts, endothelial cells, and smooth muscle cells.
Synonyms
Fibroblast Growth Factors
-
Uniprot ID
NP_001392443
Sequence Note
Ala2-Ser155
-
-
Instruction
-
Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
-
Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 2 weeks under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
-
-
Image
![SDS-PAGE analysis of recombinant swine FGF-2.]()
SDS-PAGE analysis of recombinant swine FGF-2.
1/1 -
Review
Help others learn more about this product. Use the link below to share your experience.
-
Publication
There are currently no publications. Use the link below to let us know.
-
Datasheet & Documents
1/1
Disclaimer：For Research Use or Further Manufacturing Only.
最後更新：
