Leadgene Leadgene
SUMO Protease (ULP1) ELISA Kit
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  • Overview


    SUMO Protease (ULP1, Ubiquitin-like-specific protease 1) is a highly active cysteine protease derived from Saccharomyces cerevisiae. ULP1 protease specifically recognizes the tertiary structure of SUMO rather than an amino acid sequence. It has often been used as a biotechnological tool for cleavage affinity purification tags such as ubiquitin-like (UBL) protein, and SUMO from fusion proteins. Therefore, detection of ULP1 Protease is important for evaluating the level of ULP1 Protease in samples.
    Leadgene® SUMO Protease (ULP1) ELISA kit is an enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of ULP1 Protease level in cleavage sample solution. The SUMO Protease (ULP1) ELISA kit is for research use only (RUO). Not suitable for use in diagnostic or therapeutic procedures.

    Test Principle

    SUMO Protease (ULP1) ELISA kit is used to detect ULP1 Protease in samples by sandwich ELISA method. This assay uses microplate pre-coated with mouse anti-ULP1 Protease monoclonal antibody to the solid phase. ULP1 Protease in the samples conjugates on solid phase and then react with the HRP conjugated mouse anti-ULP1 Protease monoclonal antibody. Subsequent wash steps will residual unbound antibody. After incubation with substrate solution, the reaction is determined by the absorbance at 450 nm. Quantification of ULP1 Protease level is accomplished by comparing the absorbance with standard curve.

  • Specifications
    • Reactivity

      Saccharomyces cerevisiae


      Sandwich ELISA analysis

    • Detection Method



      Limit of detection (LoD): 0.0135 ng/mL
      Limit of quantification (LoQ): 0.0451 ng/mL.

    • Specificity

      SUMO Protease

      Assay Range

      1.5625-100 ng/mL

    • Sample Type

      Samples contain SUMO Protease (ULP1)

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at 2-8°C for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • 2-8℃ for unopened product.

      Please refer to product manual for storage constructions.

  • Image
    Typical data The following data are for demonstration only

    Typical data
    The following data are for demonstration only

    Reagents provided and reconstitution

    Reagents provided and reconstitution

    An example of orientation of standards, blanks and samples in the stripwells microplate

    An example of orientation of standards, blanks and samples in the stripwells microplate

    Total   (3)
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  • Datasheet & Documents

Disclaimer:For Research Use or Further Manufacturing Only.

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