SUMO Protease (ULP1, Ubiquitin-like-specific protease 1) is a highly active cysteine protease derived from Saccharomyces cerevisiae. It has often been used as a biotechnological tool for cleavage affinity purification tags such as ubiquitinlike (UBL) protein, and SUMO from fusion proteins. ULP1 protease specifically recognizes the tertiary structure of SUMO rather than an amino acid sequence. ULP1 protease has a His-tag for easy removal from a cleavage reaction by using nickel affinity resins. Notably, the cleavage reactions are available in a buffer
containing 2 M urea.
- SUMO Protease (ULP1) protease: target protein ratio of 1U:2 µg is used for most fusion protein cleavage. Cleavage efficiency may differ based on structure and properties of each target protein, we recommend testing several enzyme-to-substrate ratios, temperatures, and incubation times.
- SUMO Protease (ULP1) reactions can be performed in a buffer containing 2M urea.
- SUMO Protease (ULP1) reactions can be performed in a buffer which is optimal for the target protein. Salts (e.g., NaCl) can be added to 300 mM for cleavage efficiency evaluation.