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SUMO Protease (ULP1) (Active)
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  • Overview


    SUMO Protease (ULP1, Ubiquitin-like-specific protease 1) is a highly active cysteine protease derived from Saccharomyces cerevisiae. It has often been used as a biotechnological tool for cleavage affinity purification tags such as ubiquitinlike (UBL) protein, and SUMO from fusion proteins. ULP1 protease specifically recognizes the tertiary structure of SUMO rather than an amino acid sequence. ULP1 protease has a His-tag for easy removal from a cleavage reaction by using nickel affinity resins. Notably, the cleavage reactions are available in a buffer
    containing 2 M urea.

    Product Note

    • SUMO Protease (ULP1) protease: target protein ratio of 1U:2 µg is used for most fusion protein cleavage. Cleavage efficiency may differ based on structure and properties of each target protein, we recommend testing several enzyme-to-substrate ratios, temperatures, and incubation times.
    • SUMO Protease (ULP1) reactions can be performed in a buffer containing 2M urea.
    • SUMO Protease (ULP1) reactions can be performed in a buffer which is optimal for the target protein. Salts (e.g., NaCl) can be added to 300 mM for cleavage efficiency evaluation.
  • Specifications
    • Expression system

      Escherichia coli


      Lyophilized from a solution containing g 50 mM Tris-HCl, 100 mM NaCl, 5 mM DTT, pH 8.0.

    • Purity

      >90% as determined by SDS-PAGE analysis.

      Unit Definition

      One unit of SUMO Protease (ULP1) cleaves > 85% of 2 μg control substrate at 30°C for 1 h.

    • Endotoxin level

      <1 EU per 1 μg of the protein by the LAL method.



  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to 50 mg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.


      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SUMO Protease (ULP1) cleavage of control protein.

    SUMO Protease (ULP1) cleavage of control protein.

    SDS-PAGE analysis of recombinant SUMO Protease (ULP1)

    SDS-PAGE analysis of recombinant SUMO Protease (ULP1)

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  • Datasheet & Documents

Disclaimer:For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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