Leadgene® StablePlus™ 2X Colorimetric RT-LAMP Master Mix is an optimized master mix for reverse-transcription loop-mediated isothermal amplification (RT-LAMP) reactions. This product is a dual enzyme system, providing a rapid and sensitive detection in one pot.
The amplified products can be visually observed by a metal indicator for calcium, hydroxynaphthol blue (HNB). Positive reactions will appear a color change from violet to sky blue, while negative reactions won’t.
The StablePlus™ version contains nucleic acid stabilizing agent to protect the amplified products.
- Primer concentration
Primer concentration can be titrated between 0.25X - 1X if undesired background signal appeared.