Description
Lys-C is a serine endoproteinase that specifically cleaves at the carboxyl side of lysine residues, generating peptides with C-terminal lysine. This recombinant form, expressed in E. coli and free from contaminating proteases, retains the activity and specificity of native Lys-C, making it suitable for protein structural analysis and sequencing applications.
Product Note
- Digestion systems should avoid the use of inhibitors such as TLCK, guanidine hydrochloride, and PMSF.
- Recommended Usage:
1. Recommended buffer pH range: 9.0–9.5; optimal reaction temperature: 30–37°C. Recombinant Lys-C retains enzymatic activity in the presence of up to 4 M urea or 0.01% SDS.
2. Suggested enzyme-to-substrate ratio: 1:50 to 1:100.
3. Incubate at 37°C overnight.
