Leadgene Leadgene
Protein A Sepharose Purification Kit
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  • Overview


    Recombinant protein A were conjugated to NHS-sepharose. Protein A is a 42 kDa surface protein found in the cell wall of Staphylococcus aureus. Protein A has a high affinity to human IgG, mouse IgG and others and is therefore suitable for purification and detection of immunoglobulins. Therefore, the protein A sepharose is an efficient technique for IgG purification.
    Recombinant protein A is immobilized at over 5 mg antibody per mL 50% slurry and this kit allows a rapid and efficient affinity purification of active IgG proteins. The affinity resin allows an efficient binding of IgG proteins without the need for preliminary steps and calibrations. The affinity bound IgG proteins can be efficiently eluted from the resin by acid condition. The eluted proteins can be used for characteristic analysis.

    Product Note

    The optimal protein concentration of the cell lysate should be determined by the end user.

  • Specifications
    • Application

      Immunoprecipitation, IgG purification



    • Specificity

      Protein A has a high affinity to human IgG, mouse IgG, and others and is, therefore, suitable for purification and detecting immunoglobulins.

      Sample Type

      Cell lysate

    • Binding capacity

      The binding and elution capacity of 1 mL settled Protein A sepharose are commonly more than 1 mg of IgG proteins.

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at 2-8°C.

      Stability & Storage

      This product is stable after storage at:

      • 2-8℃ for unopened product

      Please refer to product manual for storage constructions

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  • Datasheet & Documents

Disclaimer:For Research Use or Further Manufacturing Only.

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