PNGase F

More Images

Catalog Number

LDG0017RG

Datasheet

More Documents

Loading package & price...

COA下載

• Overview

  Description

  PNGase F is an enzyme used in biochemistry and molecular biology to remove N-linked glycans from glycoproteins. By using PNGase F, researchers can enzymatically cleave between these sugar chains and asparagine residues of glycoproteins, allowing for the study of protein structure and function, particularly in glycosylation research.

  Components

  PNGase F	1 vial (400 U/μL)
  10X Glycoprotein Denaturing Buffer	1 vial (1 mL)
  10X Reaction buffer	1 vial (1 mL)
  10% NP-40	1 vial (1 mL)

  • 10X Glycoprotein Denaturing Buffer

    1 vial (400 U/μL)

    1 vial (1 mL)

  • 10X Reaction buffer

    1 vial (400 U/μL)

    1 vial (1 mL)

  • 10% NP-40

    1 vial (400 U/μL)

    1 vial (1 mL)

• Specifications

  • Expression system

    Escherichia coli

    Concentration

    400 U /μL

  • Buffer

    20mM Tris-HCl, 50mM NaCl, 5mM EDTA, pH 7.5

    Purity

    >95% as determined by SDS-PAGE analysis.

  • Unit Definition

    One unit is defined as the amount of enzyme required to remove > 95% of the carbohydrate from 10 µg of denatured RNase B in 1 hour at 37°C in a total reaction volume of 10 µl.

    Endotoxin level

    <1 EU per 1 μg of the protein by the LAL method.

  • Form

    Liquid

• Instruction

  • Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    Stability & Storage

    This product is stable after storage at:

    • -20°C for -80°C long-term storage under sterile conditions.

    Avoid repeated free-thaw cycles.

• Image

  The standard assay was performed by incubating 1 unit of PNGase F and 10 µg of RNase B under the above conditions.
  SDS-PAGE analysis of RNase B digested with LeadGMP® PNGase F .
  Lane 1: Uncut, Lane 2: 0.01 U, Lane 3: 0.005 U, Lane 4: 0.0033 U, Lane 5: 0.0025, Lane 6: 0.002 U

  SDS-PAGE analysis of recombinant PNGase F

  1

  /

  2

  Total   (2)

• Review

  

  Help others learn more about this product. Use the link below to share your experience.

  Submit a Review

• Publication

  

  There are currently no publications. Use the link below to let us know.

  Submit a Publication

• Datasheet & Documents

  Datasheet

  MSDS

  Manual

  1

  /

  2

Disclaimer：For Research Use or Further Manufacturing Only.