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Leadgene Leadgene
Ni-NTA Sepharose Purification Kit
Catalog Number

LDG0016RD

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  • Overview

    Description

    Affinity purified Ni-NTA is conjugated to NHS-sepharose. It is an efficient technique for isolating recombinant proteins or other proteins. The Ni-NTA sepharose is specifically designed for the purification of recombinant proteins fused to the 6 x histidine (6 x His) tag expressed in bacteria, insects, and mammalian cells. The sepharose is high affinity and selectivity for recombinant fusion proteins that are tagged with six tandem histidine residues. The Ni-NTA resin can be used to purify 6 x His tagged proteins under native and denaturing conditions. Proteins bound to the resin can be eluted with low pH buffer or competition with imidazole or histidine.

    Product Note

    The optimal protein concentration of the cell lysate should be determined by the end user.

  • Specifications
    • Application

      Immunoprecipitation, Protein purification

      Conjugation

      Sepharose

    • Specificity

      Recognizes recombinant proteins fused to the 6 x histidine (6 x His) tag expressed in bacteria, insects, and mammalian cells.

      Sample Type

      Cell lysate

    • Binding capacity

      The binding and elution capacity of 1 mL settled Ni-NTA sepharose are commonly more than 5 mg of 6 x His fusion protein.

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at 2-8°C.

      Stability & Storage

      This product is stable after storage at:

      • 2-8℃ for unopened product

      Please refer to product manual for storage constructions

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  • Datasheet & Documents

Disclaimer:For Research Use or Further Manufacturing Only.

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