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Species of Origin
Mouse
Expression system
CHO
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Affinity Tag
His Tag (C-term)
Buffer
Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
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Purity
>95% as determined by SDS-PAGE analysis.
Molecular weight
The protein has a calculated MW of 26.14 kDa.
The protein migrates as 35-50 kDa under reducing condition (SDS-PAGE analysis). -
Activity
Measured by its ability to inhibit TNF-alpha mediated cytotoxicity in L-929 mouse fibroblast cells in the presence of actinomycin D and 0.25 ng/mL recombinant mouse TNF-alpha, with the ED50 being < 0.8 µg/mL.
Endotoxin level
<0.1 EU per 1 μg of the protein by the LAL method.
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Form
Lyophilized
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Specifications
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Background
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Background
TNFR-2, or Tumor Necrosis Factor Receptor 2, is a transmembrane protein found on the surface of various cell types, including immune cells and endothelial cells. It serves as a receptor for Tumor Necrosis Factor-alpha (TNF-alpha), a cytokine involved in inflammation and immune regulation. TNFR-2 signaling modulates cell survival, proliferation, and immune responses, playing a critical role in tissue homeostasis and host defense. Dysregulation of TNFR-2 has been implicated in autoimmune diseases, inflammatory disorders, and cancer. Understanding the molecular mechanisms underlying TNFR-2 signaling is essential for developing targeted therapies to modulate immune responses and treat associated diseases.
Synonyms
Tumor necrosis factor receptor 2 (TNF-R2), Tumor necrosis factor receptor type II (TNF-RII; TNFR-II), p75, p80 TNF-alpha receptor, CD120b
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Uniprot ID
P25119
Sequence Note
Met1-Gly258
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Instruction
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Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
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Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
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Image
![Activity analysis of recombinant Mouse TNFR-2, His Tag, CHO. Measured by its ability to inhibit TNF-alpha mediated cytotoxicity in L-929 mouse fibroblast cells in the presence of actinomycin D and 0.25 ng/mL recombinant mouse TNF-alpha, with the ED50 being < 0.8 µg/mL.]()
Activity analysis of recombinant Mouse TNFR-2, His Tag, CHO.
Measured by its ability to inhibit TNF-alpha mediated cytotoxicity in L-929 mouse fibroblast cells in the presence of actinomycin D and 0.25 ng/mL recombinant mouse TNF-alpha, with the ED50 being < 0.8 µg/mL.![SDS-PAGE analysis of recombinant Mouse TNFR-2.]()
SDS-PAGE analysis of recombinant Mouse TNFR-2.
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Review
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Publication
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Datasheet & Documents
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Disclaimer：For Research Use or Further Manufacturing Only.
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