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Leadgene Leadgene
Mouse IL-36 Gamma, His Tag, E. coli
Catalog Number

LDG058PME

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  • Specifications
    • Species of Origin

      Mouse

      Expression system

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Buffer

      Lyophilized from a 0.2 µm filtered solution containing 500 mM NaCl, pH 7.4.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 18.27 kDa.
      The protein migrates about 17 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to induce IL-6 secretion in 3T3 cells. The ED₅₀ for this effect is <15 ng/mL. The specific activity of recombinant mouse IL-36 gamma is > 6 x 10^⁴ IU/mg.

      Endotoxin level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      Interleukin 36 gamma (IL-36 gamma) is a 17.65 kDa cytokine with 159 amino acid residues. IL-36 gamma is a member of the IL-1 family and is mainly expressed in keratinocytes, bronchial epithelial cells, macrophages, and monocyte. This cytokine responds to multiple pathogen-associated molecular patterns (PAMPs) and participates in the inflammatory response. In addition, it also induces the production of pro-inflammatory cytokines like IL-12, IL-1β, IL-6, TNF-α, and IL-23.

      Synonyms

      Interleukin-36 gamma, Interleukin-1 family member 9, IL-1F9

    • Uniprot ID

      #Q8R460

      Sequence Note

      Gly13-Ser164

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant mouse IL-36 gamma.

    SDS-PAGE analysis of recombinant mouse IL-36 gamma.

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  • Datasheet & Documents
    MSDS
    Manual
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