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Leadgene Leadgene
Mouse IL-34, His Tag, E. coli
Catalog Number

LDG056PME

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  • Specifications
    • Species of Origin

      Mouse

      Expression system

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 25.52 kDa.
      The protein migrates about 25 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to induce proliferation in NFS-60 cells. The ED₅₀ for this effect is <30 ng/mL. The specific activity of recombinant mouse IL-34 is > 3.3 x 10⁴ IU/mg.

      Endotoxin level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      Interleukin 34 (IL-34) is a 23.98 kDa cytokine with 216 amino acid residues. IL-34 is a ligand of the colony-stimulating factor 1 receptor (CSF-1R) and is mainly secreted from the adrenal, frontal lobe, and cortex. IL-34 play a vital role in the proliferation, survival, differentiation, and migration of myeloid cells. These functions are mediated by IL-33 activating ERK1 /2, JAK, and PI3K/AKT signal pathways.

      Synonyms

      Interleukin-34, IL-34

    • Uniprot ID

      #Q8R1R4

      Sequence Note

      Asn21-Gln218

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant mouse IL-34.

    SDS-PAGE analysis of recombinant mouse IL-34.

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  • Datasheet & Documents
    MSDS
    Manual
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