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Species of Origin
Mouse
Expression system
Escherichia coli
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Affinity Tag
His Tag (C-term)
Buffer
Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
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Purity
>95% as determined by SDS-PAGE analysis.
Molecular weight
The protein has a calculated MW of 16.04 kDa.
The protein migrates as 17 kDa under reducing condition (SDS-PAGE analysis). -
Activity
Measure by its ability to induce NFS-60 cells proliferation. The ED₅₀ for this effect is <85 pg/mL. The specific activity of recombinant mouse IL-3 is approximately >1x 10⁷ IU/mg.
Endotoxin level
<0.1 EU per 1 μg of the protein by the LAL method.
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Form
Lyophilized
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Specifications
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Background
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Background
Interleukin-3 (IL-3) is a pleiotropic cytokine which can stimulates the survival, differentiation and proliferation of committed progenitor cells, including megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. IL-3 also enhances phagocytosis and antibody-mediated cellular cytotoxicity. IL-3 binds to IL-3R (IL-3 receptor), which is composed of a unique α subunit (IL-3Rα) and a common β-subunit (βc).
Synonyms
Interleukin-3, Hematopoietic Growth Factors, Mast cell Growth Factors , MCGF, Multipotential colony-stimulating factor, P-cell-stimulating factor
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Uniprot ID
P01586
Sequence Note
Asp33-Cys166
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Instruction
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Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
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Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
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Image
![HPLC analysis of recombinant Mouse IL-3, His Tag, E. coli. The purity of Mouse IL-3 Protein is greater than 95% as determined by SEC-HPLC.]()
HPLC analysis of recombinant Mouse IL-3, His Tag, E. coli.
The purity of Mouse IL-3 Protein is greater than 95% as determined by SEC-HPLC.![SDS-PAGE analysis of recombinant mouse IL-3.]()
SDS-PAGE analysis of recombinant mouse IL-3.
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Publication
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Datasheet & Documents
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Disclaimer：For Research Use or Further Manufacturing Only.
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