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Leadgene Leadgene
Mouse IFN Gamma, His Tag, E. coli
Catalog Number

LDG066PME

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  • Specifications
    • Species of Origin

      Mouse

      Expression system

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 16.5 kDa.
      The protein migrates as 16 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to anti-viral assay in L-929 cells infected with encephalomyocarditis (EMC) virus. The ED₅₀ for this effect is <0.5 ng/mL. The specific activity of recombinant mouse IFN gamma is approximately >2 x 10⁶ IU/mg.

      Endotoxin level

      <0.01 EU per 1 µg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      The cytokine IFN gamma could protect cells from viral infections and belongs to the family of interferons. A lot of studies have shown that IFN gamma secreted by antigen triggered cell types, including T cells, naive CD4+ T cells, macrophages, dendritic cells, and B cells. IFN gamma plays an important role to trigger the macrophage act against a diverse group of microbial targets, and the pleiotropic molecule associated with antiproliferative, pro-apoptotic and antitumor mechanisms. Based on the effector cytokine considered as a major effector of immunity, it has been used in the treatment of several diseases.

      Synonyms

      Interferon gamma

    • Uniprot ID

      #P01580

      Sequence Note

      His23-Cys155

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant mouse IFN gamma.

    SDS-PAGE analysis of recombinant mouse IFN gamma.

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  • Datasheet & Documents
    MSDS
    Manual
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Disclaimer:For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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