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Leadgene Leadgene
Mouse IFN Alpha 1a, His Tag, E. coli
Catalog Number

LDG064PME

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  • Specifications
    • Species of Origin

      Mouse

      Expression system

      Escherichia coli

    • Affinity Tag

      His Tag (N-term)

      Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 19.93 kDa.
      The protein migrates as 17-25 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to protect L929 cells infected with encephalomyocarditis (EMC) virus. The ED₅₀ for this effect is <10 pg/mL. The specific activity of recombinant mouse IFN alpha 1a is > 4 x 10⁷ IU/mg.

      Endotoxin level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      Interferon Alpha 1a (IFN alpha 1a) is a leukocyte interferon,
      which is a variant of Interferon-alpha. IFN-alpha 1a is a
      18.4 kDa protein containing 166 amino acid residues. It could bind the interferon receptors that activates the signal transduction of immune responses through the Jak/STAT pathway in leukocytes and lymphoblastoid cells.

      Synonyms

      Interferon alpha-1, IFN-alpha-1

    • Uniprot ID

      #P01572

      Sequence Note

      Cys24-Lys189

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant mouse IFN Alpha 1a.

    SDS-PAGE analysis of recombinant mouse IFN Alpha 1a.

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  • Datasheet & Documents
    MSDS
    Manual
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