Leadgene Leadgene
Mouse GM-CSF, His Tag, CHO
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Catalog Number

LDG079PMM

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  • Specifications
    • Species of Origin

      Mouse

      Expression system

      CHO

    • Affinity Tag

      His Tag (N-term)

      Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

    • Purity

      >90% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated 14.94 MW of kDa.
      The protein migrates as 15-30 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measured by its ability to bind mouse CD116 in functional ELISA. Immobilized Recombinant mouse CD116 Protein at 2 μg/mL (100 μL/well) can bind Recombinant Mouse GM-CSF Protein.

      Endotoxin level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine that promotes the differentiation of hematopoietic progenitors into granulocytes and macrophages. It has therapeutic applications in cancer and inflammatory diseases.

      Synonyms

      Granulocyte-macrophage colony-stimulating factor, GM-CSF, Colony-stimulating factor, CSF

    • Uniprot ID

      P01587

      Sequence Note

      Ala18 - Lys141

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    Activity analysis of recombinant Mouse GM-CSF, His Tag, CHO. Measured by its ability to bind mouse CD116 in functional ELISA. Immobilized Recombinant mouse CD116 Protein at 2 μg/mL (100 μL/well) can bind Recombinant Mouse GM-CSF Protein.

    Activity analysis of recombinant Mouse GM-CSF, His Tag, CHO.
    Measured by its ability to bind mouse CD116 in functional ELISA. Immobilized Recombinant mouse CD116 Protein at 2 μg/mL (100 μL/well) can bind Recombinant Mouse GM-CSF Protein.

    HPLC analysis of recombinant Mouse GM-CSF, His Tag, CHO. The purity of Mouse GM-CSF Protein is greater than 95% as determined by RP-HPLC.

    HPLC analysis of recombinant Mouse GM-CSF, His Tag, CHO.
    The purity of Mouse GM-CSF Protein is greater than 95% as determined by RP-HPLC.

    SDS-PAGE analysis of recombinant Mouse GM-CSF.

    SDS-PAGE analysis of recombinant Mouse GM-CSF.

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  • Datasheet & Documents
    MSDS
    Manual
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Disclaimer:For Research Use or Further Manufacturing Only.

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