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Catalog Number

LDG040PMM

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Specifications
- Species of Origin
  
  Human
  
  Expression system
  
  CHO
- Affinity Tag
  
  His Tag (C-term)
  
  Buffer
  
  Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
- Purity
  
  >95% as determined by SDS-PAGE analysis.
  
  Molecular weight
  
  The protein has a calculated MW of 61.36 kDa.
  The protein migrates as 30-42 kDa and 53 kDa under reducing condition due to a furin-like convertase cleavage site and glycosylation (SDS-PAGE analysis).
- Activity
  
  Measured by its ability to bind human FGF-2 in functional ELISA. Immobilized Recombinant human FGF-2 Protein at 5 μg/mL (100 μL/well) can bind Recombinant Mouse Glypican 3 Protein.
  
  Endotoxin level
  
  <0.1 EU per 1 μg of the protein by the LAL method.
- Form
  
  Lyophilized
Background
- Background
  
  Glypican3 is a cell surface heparan sulfate proteoglycan involved in cell growth and differentiation. It is overexpressed in hepatocellular carcinoma, making it a biomarker and therapeutic target for liver cancer.
  
  Synonyms
  
  GPC3, Glypican-3, MXR7, OCI-5, Glypican proteoglycan 3, Intestinal protein OCI-5, GTR2-2, DGSX, SDYS, SGB, SGBS, SGBS1
- Uniprot ID
  
  Q8CFZ4
  
  Sequence Note
  
  Gln25-Met557
Instruction
- Reconstitution
  
  It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
  
  Shipping
  
  The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
- Stability & Storage
  
  This product is stable after storage at:
  - -20°C for 12 months in lyophilized state from date of receipt.
  - -20°C or -80°C for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
Image

Activity analysis of recombinant Mouse Glypican 3, His Tag, CHO.
Measured by its ability to bind human FGF-2 in functional ELISA. Immobilized Recombinant human FGF-2 Protein at 5 μg/mL (100 μL/well) can bind Recombinant Mouse Glypican 3 Protein.

HPLC analysis of recombinant Mouse Glypican 3, His Tag, CHO.
The purity of Mouse Glypican 3 Protein is greater than 90% as determined by SEC-HPLC.

SDS-PAGE analysis of recombinant mouse Glypican 3.

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Datasheet & Documents

Datasheet

MSDS

Manual

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