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Catalog Number

LDG029PMM

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Specifications
- Species of Origin
  
  Mouse
  
  Expression system
  
  HEK293
- Affinity Tag
  
  His Tag (C-term)
  
  Buffer
  
  Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
- Purity
  
  >95% as determined by SDS-PAGE analysis.
  
  Molecular weight
  
  The protein has a calculated MW of 56.5 kDa.
  The protein migrates as 60 kDa under reducing condition (SDS-PAGE analysis).
- Activity
  
  Measured by its ability to bind human FGF-2 in functional ELISA. Immobilized Recombinant human FGF-2 Protein at 5 μg/mL (100 μL/well) can bind Recombinant Mouse Glypican 1 Protein.
  
  Endotoxin level
  
  <0.1 EU per 1 μg of the protein by the LAL method.
- Form
  
  Lyophilized
Background
- Background
  
  Mouse Glypican1 (GPC1) is a cell surface heparan sulfate proteoglycan that belongs to the glypican family. It is involved in various cellular processes, including growth factor signaling, cell adhesion, and differentiation. GPC1 interacts with multiple ligands, such as Wnts, Hedgehogs, and FGFs, through its heparan sulfate chains, modulating their activities and mediating their effects on cell behavior. Additionally, GPC1 has been implicated in embryonic development, neurogenesis, and tumorigenesis. In cancer, GPC1 overexpression is associated with tumor growth, invasion, and metastasis, making it a potential diagnostic and therapeutic target. Research on mouse GPC1 provides insights into the molecular mechanisms underlying its diverse functions and its role in disease pathogenesis. Understanding GPC1 biology may lead to the development of novel therapeutic strategies for cancer and other diseases involving aberrant growth factor signaling.
  
  Synonyms
  
  Gpc1
- Uniprot ID
  
  Q9QZF2
  
  Sequence Note
  
  Met1-Ser529
Instruction
- Reconstitution
  
  It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
  
  Shipping
  
  The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
- Stability & Storage
  
  This product is stable after storage at:
  - -20°C for 12 months in lyophilized state from date of receipt.
  - -20°C or -80°C for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
Image

Activity analysis of recombinant Mouse Glypican 1, His Tag, HEK293.
Measured by its ability to bind human FGF-2 in functional ELISA. Immobilized Recombinant human FGF-2 Protein at 5 μg/mL (100 μL/well) can bind Recombinant Mouse Glypican 1 Protein.

HPLC analysis of recombinant Mouse Glypican 1, His Tag, HEK293.
The purity of Mouse Glypican 1 Protein is greater than 95% as determined by RP-HPLC.

SDS-PAGE analysis of recombinant mouse glypican 1.

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Datasheet & Documents

Datasheet

MSDS

Manual

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