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Species of Origin
Mouse
Expression system
Escherichia coli
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Affinity Tag
His Tag (C-term)
Buffer
Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
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Purity
>98% as determined by SDS-PAGE analysis.
Molecular weight
The protein has a calculated MW of 19.3 kDa.
The protein migrates as 19 kDa under reducing condition (SDS-PAGE analysis). -
Activity
Measure by its ability to induce proliferation in BaF3 cells transfected with mouse Flt-3. The ED₅₀ for this effect is <2 ng/mL.
Endotoxin level
<0.1 EU per 1 μg of the protein by the LAL method.
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Form
Lyophilized
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Specifications
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Background
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Background
Fms-related tyrosine kinase-3 ligand (Flt-3 Ligand) is a protein which is encoded by the Flt3lg gene in mouse. As an important regulator of hematopoiesis, Flt-3 ligand has a tyrosine-protein kinase activity that stimulate the proliferation of hematopoietic progenitor cells of both lymphoid and myeloid origin. Flt-3 ligand can synergistically increase the proliferation of immature progenitors with other cytokines such as GM-CSF, G-CSF, IL-3, EPO, IL-11, IL-12, IL-6 or TPO. Flt-3 ligand is expressed by a variety of hematopoietic progenitor cells including immature hematopoietic and marrow stromal cells.
Synonyms
Fms-related tyrosine kinase 3 ligand, Flt3L, SL cytokine
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Uniprot ID
#P49772
Sequence Note
Gly27-Arg188
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Instruction
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Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
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Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
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Publication
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Datasheet & Documents
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Disclaimer:For Research Use or Further Manufacturing Only.