Mouse FasL, His Tag, E. coli

Catalog Number

LDG077PME

Datasheet

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• Specifications

  • Species of Origin

    Mouse

    Expression system

    Escherichia coli

  • Affinity Tag

    His Tag (N-term)

    Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

  • Purity

    >98% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 18.27 kDa.
    The protein migrates as 17-25 kDa under reducing condition (SDS-PAGE analysis).

  • Activity

    Measure by its ability to induce apoptosis in Jurkat cells. The ED₅₀ for this effect is <1 μg /mL.

    Endotoxin level

    <0.1 EU per 1 μg of the protein by the LAL method.

  • Form

    Lyophilized

• Background

  • Background

    Fas Ligand (FasL) is a 17.34 kDa tumor necrosis factor with 152 amino acid residues. FasL is mainly expressed from lymphoid tissue and secreted to blood. Binding to its receptor, TNFRSF6/FAS, leads to induce apoptotic signal into cells. Involved in cytotoxic T-cell-mediated apoptosis, natural killer cell-mediated apoptosis and in T-cell development.

    Synonyms

    Tumor necrosis factor ligand superfamily member 6, CD95 ligand, CD95-L, Fas antigen ligand, Fas ligand, FasL

  • Uniprot ID

    #P41047

    Sequence Note

    Gln128-Leu279

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  SDS-PAGE analysis of recombinant mouse FasL.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.