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Leadgene Leadgene
Mouse CXCL7 (aa 48-109), His Tag, E. coli
Catalog Number

LDG010PME

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  • Specifications
    • Species of Origin

      Mouse

      Expression system

      Escherichia coli

    • Affinity Tag

      His Tag (N-term)

      Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 7.57 kDa.
      The protein migrates below 11 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to chemoattract human THP1 cells using a concentration range of 50-100 ng/mL.

      Endotoxin level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      C-X-C motif chemokine 7 (CXCL7) also named Pro-Platelet basic protein (PPBP), which is a chemokine of the intercrine alpha family. CXCL7 is a 8.2 kDa protein containing 74 amino acid residues. CXCL7 is expressed by the platelets, which are activated. During vascular injury, CXCL7 controls the glucose metabolism, mitogenesis and neutrophil recruitment by the interaction with CXCR2.

      Synonyms

      Chemokine subfamily B Cys-X-Cys, Platelet basic protein, Pro-platelet basic protein, Thymus chemokine 1

    • Uniprot ID

      #NP_076274

      Sequence Note

      Ile48-Ile109

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant mouse CXCL7 (aa 48-109).

    SDS-PAGE analysis of recombinant mouse CXCL7 (aa 48-109).

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  • Datasheet & Documents
    MSDS
    Manual
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