Leadgene Leadgene
Mouse CD70, His Tag, CHO
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Catalog Number

LDG053PMM

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  • Specifications
    • Species of Origin

      Mouse

      Expression system

      CHO

    • Affinity Tag

      His Tag (N-term)

      Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4

    • Purity

      >95% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 17.25 kDa.
      The protein migrates as 17-30 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measured by the ability of the immobilized protein to support the adhesion of HeLa human cervical epithelial carcinoma cells. The ED50 for this effect is <2 µg/mL.

      Endotoxin level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      CD70 is a ligand for the CD27 receptor and is involved in T-cell activation and immune regulation. It is also implicated in various cancers.

      Synonyms

      CD70 antigen, CD27 ligand, CD27-L, Tumor necrosis factor ligand superfamily member 7, CD70, CD27LG, TNFSF7, TNFSF7G, CD27L

    • Uniprot ID

      O55237

      Sequence Note

      Gln47-Pro195

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    Activity analysis of recombinant Mouse CD70, His Tag, CHO. Measured by the ability of the immobilized protein to support the adhesion of HeLa human cervical epithelial carcinoma cells. The ED50 for this effect is &lt;2 µg/mL.

    Activity analysis of recombinant Mouse CD70, His Tag, CHO.
    Measured by the ability of the immobilized protein to support the adhesion of HeLa human cervical epithelial carcinoma cells. The ED50 for this effect is <2 µg/mL.

    SDS-PAGE analysis of recombinant Mouse CD70.

    SDS-PAGE analysis of recombinant Mouse CD70.

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  • Datasheet & Documents
    MSDS
    Manual
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