Leadgene Leadgene
Mouse CD19, His Tag, CHO
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Catalog Number

LDG009PMM

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  • Specifications
    • Species of Origin

      Mouse

      Expression system

      CHO

    • Affinity Tag

      His Tag (C-term)

      Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4

    • Purity

      >95% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 30.38 kDa.
      The protein migrates as 30-42 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measured by its ability to bind anti-mouse CD19 antibody in functional ELISA. Immobilized anti-mouse CD19 antibody at 2 μg/mL (50 μL/well) can bind Recombinant Mouse CD19 Protein.

      Endotoxin level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      CD19 protein, present on the surface of B cells, acts as a key regulator of B cell development and function. It plays a crucial role in B cell receptor (BCR) signaling, promoting B cell activation, proliferation, and differentiation upon antigen recognition. CD19 also enhances B cell responsiveness to co-stimulatory signals from T cells. Due to its selective expression on B cells and absence on other immune cells, CD19 serves as a valuable target for immunotherapy in B cell malignancies such as B cell lymphomas and leukemias. Understanding CD19 biology is essential for developing targeted therapies for B cell-associated disorders.

      Synonyms

      Differentiation antigen CD19, B-lymphocyte antigen CD19

    • Uniprot ID

      P25918

      Sequence Note

      Met1-Gly287

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    Activity analysis of recombinant Mouse CD19, His Tag, CHO. Measured by its ability to bind anti-mouse CD19 antibody in functional ELISA. Immobilized anti-mouse CD19 antibody at 2 μg/mL (50 μL/well) can bind Recombinant Mouse CD19 Protein.

    Activity analysis of recombinant Mouse CD19, His Tag, CHO.
    Measured by its ability to bind anti-mouse CD19 antibody in functional ELISA. Immobilized anti-mouse CD19 antibody at 2 μg/mL (50 μL/well) can bind Recombinant Mouse CD19 Protein.

    SDS-PAGE analysis of recombinant Mouse CD19.

    SDS-PAGE analysis of recombinant Mouse CD19.

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  • Datasheet & Documents
    MSDS
    Manual
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Disclaimer:For Research Use or Further Manufacturing Only.

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