Species of Origin
His Tag (C-term)
Lyophilized from a 0.2 µm filtered solution containing 20 mM sodium carbonate, pH 4.5.
>98% as determined by SDS-PAGE analysis.
The protein has a calculated MW of 12.88 kDa.
The protein migrates as 11-17 kDa under reducing condition (SDS-PAGE analysis).
Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED₅₀ for this effect is <10 ng/mL. The specific activity of recombinant mouse BMP-4 is > 1 x 10⁵ IU/mg.
<0.1 EU per 1 μg of the protein by the LAL method.
Bone Morphogenetic Protein-4 (BMP-4) predicts a molecular mass of 13 kDa, is a vital regulatory molecule that functions throughout human development in mesoderm induction, tooth development, limb formation, bone induction, and fracture repair and is overexpressed in patients who have fibrodysplasia ossificans progressiva. BMP-4 is a critical signaling molecule required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. BMP-4 is secreted from the dorsal portion of the notochord, and it acts in concert with sonic hedgehog to establish a dorsal-ventral axis for the differentiation of later structures.
Bone morphogenetic protein 4, BMP-4, Bone morphogenetic protein 2B, BMP-2B
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
SDS-PAGE analysis of recombinant mouse BMP-4.1/1
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Datasheet & Documents1/1
Disclaimer：For Research Use Only. Not for use in diagnostic or therapeutic procedures.