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LEADSPHERE® Proteinase K
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Catalog Number

LDG0001RG

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  • Overview

    Description

    Proteinase K is a non-specific serine protease that belongs to the subtilisin family with an active site catalytic triad Asp39-His69-Ser224. It is useful for the general digestion of peptide bonds, consequently used in broad applications in the biology experiments such as isolation of genomic DNA and plasmid, isolation of RNA, inactivation of RNases, DNases, and enzymes in reactions.
    LEADSPHERE® Proteinase K sphere is competent in the digestion of samples in 300-400 μL, such as saliva. In addition, LEADSPHERE® Proteinase K is generated with an ISO13485 quality management system specifically for medical devices.

    Product Note

    Please fine-tune the input sample volume to find the optimal condition for your assay.

    Components

    Package

    Items

    Quantity

    50 tablets

    LEADSPHERE® Proteinase K

    50 tablets

    Proteinase K Diluent Buffer

    1 vial (100 mL)

    • 50 tablets

      Items

      LEADSPHERE® Proteinase K

      Quantity

      50 tablets

      Items

      Proteinase K Diluent Buffer

      Quantity

      1 vial (100 mL)

  • Specifications
    • Application

      Sample preparation

      Purity

      >95% as determined by SDS-PAGE analysis.

    • Reaction Condition

      One tablet for one reaction, and one reaction can digest 16 μg BSA incubated with
      Proteinase K for 1 hour at 37o C.

      Form

      Lyophilized

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • 4-30°C for 12 months under sterile conditions from date of receipt.

      Keep dry.

  • Image
    Lyophilized LEADSPHERE® Proteinase K

    Lyophilized LEADSPHERE® Proteinase K

    Lyophilized LEADSPHERE® Proteinase K

    Lyophilized LEADSPHERE® Proteinase K

    LEADSPHERE® Proteinase K pretreatment produces sensitive detection in RT-qPCR assay.

    LEADSPHERE® Proteinase K pretreatment produces sensitive detection in RT-qPCR assay.

    LEADSPHERE™ Proteinase K completely digests bovine serum albumin (BSA). BSA was mixed with Proteinase K at 37 oC for an hour, and subsequently analyzed by SDS-PAGE. Lane 1. 2 μg of BSA Lane 2. 16 μg of BSA with 0.01 μg of Proteinase K Lane 3. 16 μg of BSA with 0.1 μg of Proteinase K Lane 4. 16 μg of BSA with 1 μg of Proteinase K

    LEADSPHERE™ Proteinase K completely digests bovine serum albumin (BSA).
    BSA was mixed with Proteinase K at 37 oC for an hour, and subsequently analyzed by SDS-PAGE. Lane 1. 2 μg of BSA Lane 2. 16 μg of BSA with 0.01 μg of Proteinase K Lane 3. 16 μg of BSA with 0.1 μg of Proteinase K Lane 4. 16 μg of BSA with 1 μg of Proteinase K

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  • Datasheet & Documents

Disclaimer:For Research Use or Further Manufacturing Only.

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