LeadGMP® T7 RNA Polymerase

Catalog Number

LDG005R-GMP

Datasheet

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• Overview

  Description

  Bacteriophage T7 RNA Polymerase is a DNA-dependent RNA polymerase with high specificity for the T7 promoter. This enzyme catalyzes the 5’→3’ synthesis of RNA from DNA downstream from its promoter.

  Components

  Package	Items	Quantity
  25,000 U	T7 RNA Polymerase (200 U/μL)	1 vial (25,000 U)
  	10× RNA Polymerase reaction buffer	1 vial (1 mL)
  	100 mM DTT	1 vial (1 mL)

  • 25,000 U

    Items

    T7 RNA Polymerase (200 U/μL)

    Quantity

    1 vial (25,000 U)

    Items

    10× RNA Polymerase reaction buffer

    Quantity

    1 vial (1 mL)

    Items

    100 mM DTT

    Quantity

    1 vial (1 mL)

• Specifications

  • Expression system

    Escherichia coli

    Buffer

    T7 RNA Polymerase is supplied in 100 mM Tris-HCl (pH 7.9), 20 mM KCl, 1 mM DTT, 1 mM EDTA, 0.1% Triton® X-100 and 50% (v/v) glycerol.

  • Purity

    >98% as determined by SDS-PAGE analysis.

    Unit Definition

    One unit is defined as the amount of the enzyme incorporates 1 nmol of ATP into acid-insoluble product in 1 hour at 37°C.

  • Reaction Condition

    1× RNA Polymerase Reaction Buffer, supplemented with 0.5 mM each ATP, UTP, GTP, CTP, and DNA template containing the T7 RNA Polymerase promoter. Incubate at 37°C.
    10× RNA Polymerase Reaction Buffer: 400 mM Tris-HCl (pH 8.0), 60 mM MgCl₂, and 20 mM spermidine.

• Instruction

  • Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    Stability & Storage

    This product is stable after storage at:

    • -20°C or -80°C for 12 months under sterile conditions from date of receipt.
    • For optimal storage, aliquot the enzyme, reaction buffer and DTT reagent into smaller quantities and store at recommended temperature.
    • For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

  • Manufacturing specifications

    LeadGMP® recombinant proteins are manufactured in ISO 13485:2016 and GMP certified facility. The processes include:

    • Animal-free reagent and laboratory
    • Manufactured and tested under GMP guideline
    • Testing and traceability of raw material
    • Records of the maintenance and equipment calibration
    • Personnel training records
    • Batch-to-batch consistency
    • Documentation of QA control and process changes
    • Manufactured and tested under an ISO 13485:2016 certified quality management system
    • Stability monitor of product shelf-life

• Image

  

  SDS-PAGE analysis of recombinant T7 RNA Polymerase.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.