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Leadgene Leadgene
LeadGMP® Murine RNase Inhibitor
Catalog Number

LDG006R-GMP

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  • Specifications
    • Species of Origin

      Mouse

      Expression system

      Escherichia coli

    • Concentration

      40 U/ μL

      Buffer

      20 mM HEPES-KOH, 50 mM KCl, 8 mM DTT, 50% glycerol, pH 7.6.

    • Purity

      >95% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 50 kDa.
      The protein migrates as 45 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      One unit is defined as the amount of Murine RNase Inhibitor required to inhibit the activity of 5 ng of RNase A by 50%.

      Endotoxin level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Mycoplasma

      Not detected

      Form

      Liquid

  • Background
    • Background

      Murine RNase Inhibitor is a recombinant protein derived from mice, expressed in E. coli. It binds noncovalently to RNases A, B, and C in a 1:1 ratio, effectively inhibiting their activity and protecting RNA from degradation. This inhibitor is commonly used in molecular biology applications to maintain RNA integrity during experiments.

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • -20°C for long-term storage under sterile conditions. Avoid repeated free-thaw cycles.
    • Manufacturing specifications

      LeadGMP® recombinant proteins are manufactured in ISO 13485:2016 and GMP certified facility. The processes include:

      • Animal-free reagent and laboratory
      • Manufactured and tested under GMP guideline
      • Testing and traceability of raw material
      • Records of the maintenance and equipment calibration
      • Personnel training records
      • Batch-to-batch consistency
      • Documentation of QA control and process changes
      • Manufactured and tested under an ISO 13485:2016 certified quality management system
      • Stability monitor of product shelf-life
  • Image
    SDS-PAGE analysis of LeadGMP® Murine RNase Inhibitor.

    SDS-PAGE analysis of LeadGMP® Murine RNase Inhibitor.

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  • Datasheet & Documents
    MSDS
    Manual
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