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Species of Origin
Mouse
Expression system
Escherichia coli
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Concentration
40 U/ μL
Buffer
20 mM HEPES-KOH, 50 mM KCl, 8 mM DTT, 50% glycerol, pH 7.6.
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Purity
>95% as determined by SDS-PAGE analysis.
Molecular weight
The protein has a calculated MW of 50 kDa.
The protein migrates as 45 kDa under reducing condition (SDS-PAGE analysis). -
Activity
One unit is defined as the amount of Murine RNase Inhibitor required to inhibit the activity of 5 ng of RNase A by 50%.
Endotoxin level
<0.1 EU per 1 μg of the protein by the LAL method.
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Mycoplasma
Not detected
Form
Liquid
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Specifications
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Background
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Background
Murine RNase Inhibitor is a recombinant protein derived from mice, expressed in E. coli. It binds noncovalently to RNases A, B, and C in a 1:1 ratio, effectively inhibiting their activity and protecting RNA from degradation. This inhibitor is commonly used in molecular biology applications to maintain RNA integrity during experiments.
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Instruction
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Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
Stability & Storage
This product is stable after storage at:
- -20°C for long-term storage under sterile conditions. Avoid repeated free-thaw cycles.
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Manufacturing specifications
LeadGMP® recombinant proteins are manufactured in ISO 13485:2016 and GMP certified facility. The processes include:
- Animal-free reagent and laboratory
- Manufactured and tested under GMP guideline
- Testing and traceability of raw material
- Records of the maintenance and equipment calibration
- Personnel training records
- Batch-to-batch consistency
- Documentation of QA control and process changes
- Manufactured and tested under an ISO 13485:2016 certified quality management system
- Stability monitor of product shelf-life
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Publication
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Datasheet & Documents
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Disclaimer:For Research Use or Further Manufacturing Only.