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LeadGMP® Human IL-21 Protein, His Tag, E. coli
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  • Specifications
    • Species of Origin


      Expression system

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)


      Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

    • Purity

      >97% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 16.2 kDa.
      The protein migrates as 15-19 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to enhance IFN gamma secretion in NK-92 cells. The ED₅₀ for this effect is <10 ng/mL.

      Endotoxin level

      <0.05 EU per 1 µg of the protein by the LAL method.

    • Mycoplasma

      Not detected



  • Background
    • Background

      Interleukin-21 (IL-21) belongs to the IL-15/IL-21 family, which exerts pleiotropic immune regulations. IL-21 produced primarily by natural killer T (NKT) cells, T follicular helper (TFH) cells and TH17 cells. As a pleiotropic cytokine, IL-21 has been shown to regulate both innate and humoral immunity. It has potent inhibitory activity towards the activation and maturation of granulocyte-macrophage colony-stimulating factor (GM-CSF)-induced dendritic cells (DCs). In B cells, IL-21 has a major role in the development of immunoglobulin responses. In T cells, it is required to facilitate the functional differentiation of several CD4+ T cell subsets. In addition, the ability of IL-21 to enhance the cytotoxic activity of both CD8+ T cells and NK cells makes it as a potential antitumor agent.


      Interleukin-21, Za11

    • Uniprot ID


      Sequence Note


  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 0.5 mg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.


      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

      Manufacturing specifications

      LeadGMP® recombinant proteins are manufactured in ISO 13485:2016 and GMP certified facility. The processes include:

      • Animal-free reagent and laboratory
      • Manufactured and tested under GMP guideline
      • Testing and traceability of raw material
      • Records of the maintenance and equipment calibration
      • Personnel training records
      • Batch-to-batch consistency
      • Documentation of QA control and process changes
      • Manufactured and tested under an ISO 13485:2016 certified quality management system
      • Stability monitor of product shelf-life
  • Image
    LeadGMP® Human IL-21 Protein, His Tag, E. coli (LDG011PHE-GMP) enhances IFN gamma secretion in NK-92 cells, with the ED50 &lt;10 ng/mL

    LeadGMP® Human IL-21 Protein, His Tag, E. coli (LDG011PHE-GMP) enhances IFN gamma secretion in NK-92 cells, with the ED50 <10 ng/mL

    SDS-PAGE analysis of LeadGMP® IL-21, Human

    SDS-PAGE analysis of LeadGMP® IL-21, Human

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  • Datasheet & Documents

Disclaimer:For Research Use or Further Manufacturing Only.

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