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LeadGMP® Human IL-2 Protein, His Tag, E. coli
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Catalog Number

LDG003PHE-GMP

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  • Specifications
    • Species of Origin

      Human

      Expression system

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Buffer

      Lyophilized from a 0.2 µm filtered solution of NaPi buffer, 0.018% SDS, pH 7.5.

    • Purity

      >97% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 16.4 kDa.
      The protein migrates as 13 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to induce proliferation in CTLL-2 cells. The ED₅₀ for this effect is <3 ng/mL. The specific activity of recombinant human IL-2 is >5 x 10⁶ IU/mg, which is calibrated against the human IL-2 WHO International Standard (NIBSC code: 86/500).

      Endotoxin level

      <0.05 EU per 1 µg of the protein by the LAL method.

    • Mycoplasma

      Not detected

      Form

      Lyophilized

  • Background
    • Background

      Interleukin-2 (IL-2) is a 15.5 kDa four bundle, immuno-modulatory cytokine which is expressed predominately by antigen-simulated CD4+ T cells, CD8+ T cells, natural killer (NK) cells, and dendritic cells (DC). IL-2 plays a fundamental role in promoting NK cell proliferation, cytotoxicity, and B cells differentiation. IL-2 modulate T cell differentiation programs, promoting naïve CD4+ T cell differentiation into T helper-1 (Th1) and T helper-2 (Th2) cells.

      Synonyms

      Interleukin-2, T-cell growth factor , TCGF

    • Uniprot ID

      P60568

      Sequence Note

      Ala21-Thr153

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 0.5 mg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

      Manufacturing specifications

      LeadGMP® recombinant proteins are manufactured in ISO 13485:2016 and GMP certified facility. The processes include:

      • Animal-free reagent and laboratory
      • Manufactured and tested under GMP guideline
      • Testing and traceability of raw material
      • Records of the maintenance and equipment calibration
      • Personnel training records
      • Batch-to-batch consistency
      • Documentation of QA control and process changes
      • Manufactured and tested under an ISO 13485:2016 certified quality management system
      • Stability monitor of product shelf-life
  • Image
    LeadGMP® Human IL-2 Protein, His Tag, E. coli (LDG003PHE-GMP) induces CTLL-2 cell proliferation, with the ED50 &lt; 3.0 ng/mL.

    LeadGMP® Human IL-2 Protein, His Tag, E. coli (LDG003PHE-GMP) induces CTLL-2 cell proliferation, with the ED50 < 3.0 ng/mL.

    SDS-PAGE analysis of LeadGMP® IL-2, Human

    SDS-PAGE analysis of LeadGMP® IL-2, Human

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  • Datasheet & Documents
    MSDS
    Manual
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