LeadGMP® Endoglycosidase H (Endo H)

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Catalog Number

LDG002R-GMP

Datasheet

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• Overview

  Product Note

  • After thawing, the buffers may crystallize, which is a normal occurrence. Warm crystallized buffer until the salt crystals return to solution. Ensure that your components return to RT before use in the assay.
  • Please fine-tune the input sample volume to find the optimal condition for your assay.
  • Once optimize for the cleavage condition, the cleavage reactions can be scaled up to cleave a large amount of the target fusion protein.

  Components

  Items	Quantity
  LeadGMP® Endoglycosidase H (Endo H) (500 U/μL)	1 vial (50,000 U)
  10× Glycoprotein Denaturing Buffer	1 vial (1 mL)
  10× Reaction buffer	1 vial (1 mL)

  • LeadGMP® Endoglycosidase H (Endo H) (500 U/μL)

    Quantity

    1 vial (50,000 U)

  • 10× Glycoprotein Denaturing Buffer

    Quantity

    1 vial (1 mL)

  • 10× Reaction buffer

    Quantity

    1 vial (1 mL)

• Specifications

  • Species of Origin

    Streptomyces plicatus

    Expression system

    Escherichia coli

  • Concentration

    500 U/μL

    Buffer

    20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH 7.5

  • Purity

    >95% as determined by SDS-PAGE analysis.

    Activity

    One unit of Endoglycosidase H cleaves > 95% of the carbohydrate from 10 μg of denatured RNase B in a total reaction volume of 10 μL at 37°C for 1 h.

  • Endotoxin level

    <0.05 EU per 1 µg of the protein by the LAL method.

    Mycoplasma

    Not detected

  • Form

    Liquid

• Background

  • Background

    Protein glycosylation is a complex posttranslational modification that manipulates biological activity such as protein folding, intracellular trafficking, stability, and half-life, affecting protein function. Endoglycosidase H is a glycosidase that cleaves asparagine-linked oligomannose and hybrid, but not glycan complex, from N-linked glycoproteins. It hydrolyses the bond connecting the two N-acetylglucosamine residues comprising the chitobiose core, leaving an N-acetylglucosamine residue on the asparagine.

• Instruction

  • Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    Stability & Storage

    This product is stable after storage at:

    • -20°C for long-term storage under sterile conditions. Avoid repeated free-thaw cycles.

  • Manufacturing specifications

    LeadGMP® recombinant proteins are manufactured in ISO 13485:2016 and GMP certified facility. The processes include:

    • Animal-free reagent and laboratory
    • Manufactured and tested under GMP guideline
    • Testing and traceability of raw material
    • Records of the maintenance and equipment calibration
    • Personnel training records
    • Batch-to-batch consistency
    • Documentation of QA control and process changes
    • Manufactured and tested under an ISO 13485:2016 certified quality management system
    • Stability monitor of product shelf-life

• Image

  Endo H cleaves high mannose and hybrid-type glycans.

  The standard assay was performed by incubating 1 unit of Endo H and 10 µg of RNase B under the above conditions. SDS-PAGE analysis of RNase B digested with Endoglycosidase H.

  SDS-PAGE analysis of recombinant Endoglycosidase H

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.

最後更新：2025.12.23