Components
LeadGMP® Endoglycosidase H (Endo H) (500 U/μL)
Quantity
1 vial (50,000 U)
10× Glycoprotein Denaturing Buffer
Quantity
1 vial (1 mL)
10× Reaction buffer
Quantity
1 vial (1 mL)
Components
LeadGMP® Endoglycosidase H (Endo H) (500 U/μL)
Quantity
1 vial (50,000 U)
10× Glycoprotein Denaturing Buffer
Quantity
1 vial (1 mL)
10× Reaction buffer
Quantity
1 vial (1 mL)
Species of Origin
Streptomyces plicatus
Expression system
Escherichia coli
Concentration
500 U/μL
Buffer
20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH 7.5
Purity
>95% as determined by SDS-PAGE analysis.
Activity
One unit of Endoglycosidase H cleaves > 95% of the carbohydrate from 10 μg of denatured RNase B in a total reaction volume of 10 μL at 37°C for 1 h.
Endotoxin level
<0.05 EU per 1 µg of the protein by the LAL method.
Mycoplasma
Not detected
Form
Liquid
Background
Protein glycosylation is a complex posttranslational modification that manipulates biological activity such as protein folding, intracellular trafficking, stability, and half-life, affecting protein function. Endoglycosidase H is a glycosidase that cleaves asparagine-linked oligomannose and hybrid, but not glycan complex, from N-linked glycoproteins. It hydrolyses the bond connecting the two N-acetylglucosamine residues comprising the chitobiose core, leaving an N-acetylglucosamine residue on the asparagine.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
Stability & Storage
This product is stable after storage at:
Manufacturing specifications
LeadGMP® recombinant proteins are manufactured in ISO 13485:2016 and GMP certified facility. The processes include:
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