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Catalog Number

LDG002R-GMP

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Overview
Product Note
- After thawing, the buffers may crystallize, which is a normal occurrence. Warm crystallized buffer until the salt crystals return to solution. Ensure that your components return to RT before use in the assay.
- Please fine-tune the input sample volume to find the optimal condition for your assay.
- Once optimize for the cleavage condition, the cleavage reactions can be scaled up to cleave a large amount of the target fusion protein.
Components

Items
Quantity
LeadGMP® Endoglycosidase H (Endo H) (500 U/μL)
1 vial (50,000 U)
10× Glycoprotein Denaturing Buffer
1 vial (1 mL)
10× Reaction buffer
1 vial (1 mL)
- LeadGMP® Endoglycosidase H (Endo H) (500 U/μL)
  Quantity
  1 vial (50,000 U)
- 10× Glycoprotein Denaturing Buffer
  Quantity
  1 vial (1 mL)
- 10× Reaction buffer
  Quantity
  1 vial (1 mL)
Specifications
- Species of Origin
  
  Streptomyces plicatus
  
  Expression system
  
  Escherichia coli
- Concentration
  
  500 U/μL
  
  Buffer
  
  20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH 7.5
- Purity
  
  >95% as determined by SDS-PAGE analysis.
  
  Activity
  
  One unit of Endoglycosidase H cleaves > 95% of the carbohydrate from 10 μg of denatured RNase B in a total reaction volume of 10 μL at 37°C for 1 h.
- Endotoxin level
  
  <0.05 EU per 1 µg of the protein by the LAL method.
  
  Mycoplasma
  
  Not detected
- Form
  
  Liquid
Background
- Background
  
  Protein glycosylation is a complex posttranslational modification that manipulates biological activity such as protein folding, intracellular trafficking, stability, and half-life, affecting protein function. Endoglycosidase H is a glycosidase that cleaves asparagine-linked oligomannose and hybrid, but not glycan complex, from N-linked glycoproteins. It hydrolyses the bond connecting the two N-acetylglucosamine residues comprising the chitobiose core, leaving an N-acetylglucosamine residue on the asparagine.
Instruction
- Shipping
  
  The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
  Stability & Storage
  
  This product is stable after storage at:
  - -20°C for long-term storage under sterile conditions. Avoid repeated free-thaw cycles.
- Manufacturing specifications
  
  LeadGMP® recombinant proteins are manufactured in ISO 13485:2016 and GMP certified facility. The processes include:
  - Animal-free reagent and laboratory
  - Manufactured and tested under GMP guideline
  - Testing and traceability of raw material
  - Records of the maintenance and equipment calibration
  - Personnel training records
  - Batch-to-batch consistency
  - Documentation of QA control and process changes
  - Manufactured and tested under an ISO 13485:2016 certified quality management system
  - Stability monitor of product shelf-life
Image

Endo H cleaves high mannose and hybrid-type glycans.

The standard assay was performed by incubating 1 unit of Endo H and 10 µg of RNase B under the above conditions. SDS-PAGE analysis of RNase B digested with Endoglycosidase H.

SDS-PAGE analysis of recombinant Endoglycosidase H

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Datasheet & Documents

Datasheet

MSDS

Manual

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