Lactate Oxidase (LOX)

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Catalog Number

LDG0036RG

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• Overview

  Description

  Lactate oxidase (LOX) is an enzyme that catalyzes the oxidation of L-lactate to pyruvate and hydrogen peroxide. This flavoprotein, primarily found in bacteria and fungi, plays a crucial role in various biological processes, including energy metabolism and redox regulation. Due to its high specificity for lactate, LOX is widely utilized in biosensors for detecting lactate in clinical diagnostics, food quality control, and sports science. Its application allows for accurate and rapid quantification of lactate levels.

• Specifications

  • Expression system

    Escherichia coli

    Detection Method

    Spectrophotometry

  • Concentration

    200 U/mg or more

    Activity

    Activity can be calculated by the following formula :
    Volume activity (U/mL) ＝ ΔOD (OD test−OD blank)×Vt×df/ (34.3×1/2×t×1.0×Vs) ＝ ΔOD×0.237×df
    Weight activity (U/mg) ＝ (U/mL)×(1/C)
    Vt: Total volume (1.525 mL)
    Vs: Sample volume (0.025 mL)
    34.3: Millimolar extinction coefficient of quinoneimine dye under the assay condition (cm²/micromole)
    1/2: Factor based on the fact that one mole of H₂O₂ produced half a mole of quinoneimine dye
    t: Reaction time (15 minutes)
    1.0: Light path length (cm)
    df: Dilution factor
    C: Enzyme concentration (C mg/mL)

  • Unit Definition

    One unit causes the formation of one micromole of hydrogen peroxide (half a micromole of quinoneimine dye) per minute under the following conditions: 20 mM potassium phosphate buffer (pH 7.5), 48 mM D-L-lactate, 1.2 mM 4-aminoantipyrine, 0.76 mM EHSPT, and 2.4 U/mL peroxidase.

• Background

  • Synonyms

    LOX, LctO, Lactic oxygenase, Lactic oxidase, Lactate monooxygenase, L-lactate oxidase

• Instruction

  • Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    Stability & Storage

    This product is stable at -20°C for long-term storage under sterile conditions.
    Avoid repeated free-thaw cycles.

• Image

  pH stability of LOX.
  The enzyme powder was reconstituted by double-distilled water and treated with different pH buffer condition at 25°C for 16 hours. pH 4.0-6.0, 0.1 M Sodium citrate buffer; pH 7.5-8.0, 0.1 M Potassium phosphate buffer; pH 8.5, 0.1 M Tris-HCl buffer; pH 10.0-11.0, 0.1 M Carbonate-bicarbonate buffer.

  Thermal stability of LOX.
  The enzyme powder was reconstituted by double-distilled water and treated at different temperatures for 10 minutes. Final concentration: 10 U/mL.

  pH activity of LOX.
  The buffer conditions with various pH values were used in the reaction at 37°C. pH 4.0-6.0, 0.1 M Sodium citrate buffer; pH 7.5-8.0, 0.1 M Potassium phosphate buffer; pH 8.5, 0.1 M Tris-HCl buffer; pH 10.0-11.0, 0.1 M Carbonate-bicarbonate buffer.

  Temperature activity of LOX.
  The enzyme reactions in 20 M K-Phosphate buffer, pH 7.5, were carried out under different temperatures.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.