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  • Overview


    Interleukin-8 (IL-8) is a multifunctional chemokine produced by macrophages and several cell types such as epithelial cells, airway smooth muscle cells and endothelial cells. IL-8 is an important protein related to inflammation, that attracts neutrophils and other immune cells to the site of infection. IL-8 also participates in neutrophil chemotactic effects, mitogenesis, leukocyte activation, and calcium homeostasis.
    Leadgene® IL-8 ELISA kit is an enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of IL-8 level in human serum sample. The IL-8 ELISA kit is for research use only (RUO). Not suitable for use in diagnostic or therapeutic procedures.

    Test Principle

    IL-8 ELISA kit is used to detect IL-8 in human serum and recombinant human IL-8 proteins by sandwich ELISA method. This assay uses microplate pre-coated with mouse anti-IL-8 monoclonal antibody to the solid phase. IL-8 in the serum samples conjugates on solid phase and then react with the HRP conjugated mouse anti-IL-8 monoclonal antibody. Subsequent wash steps will residual unbound antibody. After incubation with substrate solution, the reaction is determined by the absorbance at 450 nm. Quantification of IL-8 level is accomplished by comparing the absorbance with standards curve.

  • Specifications
    • Reactivity



      Sandwich ELISA analysis

    • Detection Method



      Limit of detection (LoD): 5 pg/mL
      Limit of quantification (LoQ): 10 pg/mL

    • Specificity

      Interleukin 8. There is no significant cross reactivity in the presence of 1 μg/mL of IL-4 and IL-6.

      Assay Range

      6.25-400 pg/mL

    • Sample Type


  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at 2-8°C for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • 2-8℃ for unopened product.

      Please refer to product manual for storage constructions.

  • Image
    Typical data The following data are for demonstration only

    Typical data
    The following data are for demonstration only

    Reagents provided and reconstitution

    Reagents provided and reconstitution

    An example of orientation of standards, blanks and samples in the stripwells microplate

    An example of orientation of standards, blanks and samples in the stripwells microplate

    Total   (3)
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  • Datasheet & Documents

Disclaimer:For Research Use or Further Manufacturing Only.

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