Human Wnt-3a Surrogate, Fc Tag, HEK293

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Catalog Number

LDG168PHM

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• Specifications

  • Species of Origin

    Human

    Expression system

    HEK293

  • Affinity Tag

    Human IgG1 Fc Tag (C-term)

    Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

  • Purity

    >95% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 58.5 kDa.
    The protein migrates as 55-75 kDa under reducing condition (SDS-PAGE analysis).

  • Activity

    Measured by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED₅₀ for this effect is <20 ng/mL.

    Endotoxin level

    <0.1 EU per 1 μg of the protein by the LAL method.

  • Form

    Lyophilized

• Background

  • Background

    ​Wnt3a is a secreted glycoprotein that activates the canonical Wnt/β-catenin signaling pathway, playing a crucial role in embryonic development, tissue homeostasis, and injury repair. However, its experimental application is hindered by poor solubility and cross-reactivity with multiple Frizzled receptors. To address these challenges, engineered Wnt3a surrogate proteins have been developed. These surrogates are water-soluble and designed to specifically bind to targeted Frizzled receptors, effectively mimicking natural Wnt3a activity with enhanced precision. This specificity and improved solubility make Wnt3a surrogates valuable tools in research areas such as organoid culture, stem cell maintenance, and studies of developmental biology.

    Synonyms

    Wnt3a Surrogate​, Wingless-type MMTV integration site family member 3A Surrogate, WNT3A

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  Activity analysis of recombinant Human Wnt-3a Surrogate, Fc Tag, HEK293.
  Measured by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED₅₀ for this effect is <20 ng/mL.

  SDS-PAGE analysis of recombinant Human Wnt-3a Surrogate.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.