Human Vitronectin, His Tag, E. coli

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Catalog Number

LDG196PHE

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• Specifications

  • Species of Origin

    Human

    Expression system

    Escherichia coli

  • Affinity Tag

    His Tag (C-term)

    Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

  • Purity

    >95% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 48.43 kDa.
    The protein migrates as 47-55 kDa under reducing condition (SDS-PAGE analysis).

  • Activity

    Measured by the ability of the immobilized protein to support the adhesion of B16‑F1 mouse melanoma cells. The ED50 for this effect is <0.5 μg/mL.

    Endotoxin level

    <0.1 EU per 1 μg of the protein by the LAL method.

  • Form

    Lyophilized

• Background

  • Background

    Human Vitronectin is a multifunctional glycoprotein present in serum and the extracellular matrix. It promotes cell adhesion, migration, and tissue repair by interacting with integrins and other ECM components. Vitronectin plays critical roles in wound healing, angiogenesis, and regulation of the complement and coagulation cascades. Recombinant Vitronectin is widely used in cell culture, regenerative medicine, and cancer research as a defined substrate for cell attachment and signaling studies.

    Synonyms

    Serum spreading factor, S-protein, Complement S-protein, VTN, Epibolin Synonym, V75, VN, Vitronectin

  • Uniprot ID

    P04004

    Sequence Note

    Val62-Leu478

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  Activity analysis of recombinant Human Vitronectin, His Tag, E. coli.
  Measured by the ability of the immobilized protein to support the adhesion of B16‑F1 mouse melanoma cells. The ED50 for this effect is <0.5 μg/mL.

  SDS-PAGE analysis of recombinant human vitronectin protein.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.