Leadgene Leadgene
Human VEGF121, His Tag, E. coli
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  • Specifications
    • Species of Origin


      Expression system

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)


      Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

    • Purity

      >95% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 15.00 kDa.
      The protein migrates as 15 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to induce proliferation in HUVEC cells. The ED₅₀ for this effect is <2.5 ng/mL.

      Endotoxin level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form


  • Background
    • Background

      Vascular Endothelial Growth Factors 121(VEGF121) is one of five VEGF splice variants of 121, 145, 165, 183, 189, and 206 amino acids (aa) in length. VEGF121 is the only VEGF which lacks heparin-binding activity and freely diffusible. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1 /KDR) on endothelial cells to activate signal transduction and regulate both physiological and pathological angiogenesis.


      Vascular endothelial Growth Factors A, VEGF-A, Vascular permeability factor, VPF

    • Uniprot ID

      #PDB: 3V2A_A

      Sequence Note


  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.


      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    Human VEGF121, His Tag, E. coli (LDG060PHE) induced HUVEC cell proliferation, with the ED50 at 2.11 ng/mL.

    Human VEGF121, His Tag, E. coli (LDG060PHE) induced HUVEC cell proliferation, with the ED50 at 2.11 ng/mL.

    SDS-PAGE analysis of recombinant human VEGF121.

    SDS-PAGE analysis of recombinant human VEGF121.

    Total   (2)
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  • Datasheet & Documents

Disclaimer:For Research Use or Further Manufacturing Only.

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