Species of Origin
His Tag (C-term)
Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.
>98% as determined by SDS-PAGE analysis.
The protein has a calculated MW of 17.88 kDa.
The protein migrates as 18 kDa under reducing condition (SDS-PAGE analysis).
Measure by its ability to induce proliferation in HUVEC cells. The ED₅₀ for this effect is <6 ng/mL.
<0.1 EU per 1 μg of the protein by the LAL method.
TNF-related weak inducer of apoptosis (TWEAK) is a type II membrane protein of the tumor necrosis factor (TNF) family members, high levels expression in human brain, muscle, heart, spleen, and thymus. TWEAK is a 17.3 kDa protein containing 129 residues. TWEAK induce apoptosis through cognate receptor Fn14 activate caspase-8 and caspase-3, in HSC3 cells KATO-III gastric adenocarcinoma cells and IFN-γ-treated HT-29. TWEAK stimulate inflammatory cytokines in HT-29, A375 cells, WI-38 fibroblast sand astrocytes.
TNF-Related Weak Inducer of Apoptosis, TWEAK, APO3 ligand, Tumor necrosis factor ligand superfamily member 12, TNFL12
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
Human TWEAK, His Tag, E. coli (LDG123PHE) induced HUVEC cell proliferation, with the ED50 at 5.84 ng/mL.
SDS-PAGE analysis of recombinant human TWEAK.1/2
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Datasheet & Documents1/1
Disclaimer：For Research Use Only. Not for use in diagnostic or therapeutic procedures.