Human TPO, His Tag, E. coli

Catalog Number

LDG065PHE

Datasheet

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• Specifications

  • Species of Origin

    Human

    Expression system

    Escherichia coli

  • Affinity Tag

    His Tag (N-term)

    Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

  • Purity

    >95% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 19.6 kDa.
    The protein migrates as 20 kDa under reducing condition (SDS-PAGE analysis).

  • Endotoxin level

    <0.01 EU per 1 µg of the protein by the LAL method.

    Form

    Lyophilized

• Background

  • Background

    Thrombopoietin (TPO) is a glycoprotein that produced by the liver, kidney, marrow stroma and several other tissues. The TPO level in the blood is mostly negatively correlated with the abundance of platelets and bone marrow megakaryocytes, although multiple states of inflammation or infection, liver failure, and hematological disturbances are associated with unexpectedly high or low circulating levels of the hormone.

    Synonyms

    Thrombopoietin, C-mpl ligand , ML, Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor , MGDF, Myeloproliferative leukemia virus oncogene ligand

  • Uniprot ID

    #P40225

    Sequence Note

    Ser22-Leu195

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 2 weeks under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  SDS-PAGE analysis of recombinant human TPO.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.