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Species of Origin
Human
Expression system
CHO
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Affinity Tag
His Tag (N-term)
Buffer
Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
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Purity
>85% as determined by SDS-PAGE analysis.
Molecular weight
The protein has a calculated MW of 22.33 kDa.
The protein migrates as 25-35 kDa under reducing condition (SDS-PAGE analysis). -
Activity
Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by ATDC5 cells. The ED₅₀ for this effect is <8 μg/mL.
Form
Lyophilized
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Specifications
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Background
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Background
Human Sclerostin is a glycoprotein primarily expressed by osteocytes and plays a key role in regulating bone formation by inhibiting the Wnt signaling pathway, which is crucial for bone growth. Sclerostin acts as a negative regulator of bone mass, and its activity is of significant interest in research related to osteoporosis and other bone-related disorders. The Human Sclerostin protein, when expressed in CHO (Chinese Hamster Ovary) cells, is often tagged with a His (histidine) tag, facilitating its purification and detection in research applications. This recombinant Sclerostin-His fusion protein is commonly used in studies aimed at understanding bone metabolism and developing therapeutic strategies for enhancing bone formation.
Synonyms
Sclerostin
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Uniprot ID
Q9BQB4
Sequence Note
Gln24-Tyr213
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Instruction
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Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
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Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
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Image
![Activity analysis of recombinant Human Sclerostin, His Tag, CHO. Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by ATDC5 cells. The ED₅₀ for this effect is <8 μg/mL.]()
Activity analysis of recombinant Human Sclerostin, His Tag, CHO.
Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by ATDC5 cells. The ED₅₀ for this effect is <8 μg/mL.![SDS-PAGE analysis of recombinant human Sclerostin.]()
SDS-PAGE analysis of recombinant human Sclerostin.
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Publication
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Datasheet & Documents
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Disclaimer：For Research Use or Further Manufacturing Only.
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