Human R-Spondin 3, His-SUMO Tag, CHO

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Catalog Number

LDG199PHM

Datasheet

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• Specifications

  • Species of Origin

    Human

    Expression system

    CHO

  • Affinity Tag

    His-SUMO Tag (N-term)

    Concentration

    Please refer to the product label.

  • Buffer

    Liquid. Phosphate buffered saline, pH 7.4.

    Purity

    >90% as determined by SDS-PAGE analysis.

  • Molecular weight

    The protein has a calculated MW of 48.4 kDa.
    The protein migrates as 62-70 kDa under reducing condition (SDS-PAGE analysis).

    Activity

    R-Spondin 3 enhances BMP-2-mediated differentiation of MC3T3-E1 cells. The ED₅₀ for this effect is < 3 μg/mL.

  • Endotoxin level

    <0.1 EU per 1 μg of the protein by the LAL method.

    Form

    Liquid

• Background

  • Background

    R-Spondin 3 (RSPO3) is a secreted growth factor that enhances canonical Wnt/β-catenin signaling through interaction with LGR4/5/6 receptors and inhibition of RNF43/ZNRF3-mediated receptor turnover. RSPO3 plays essential roles in stem cell maintenance, angiogenesis, vascular development, tissue regeneration, and embryonic morphogenesis. It is widely used in organoid culture systems to support stem cell proliferation and epithelial growth. Aberrant RSPO3-mediated Wnt signaling has also been implicated in cancer progression and tumor microenvironment regulation.

    Synonyms

    R-Spondin 3, RSPO3, Roof plate-specific spondin-3, LGR-binding growth factor, PWTSR, THSD2, Protein with thrombospondin type 1 repeat, Thrombospondin type-1 domain-containing protein 2

  • Uniprot ID

    Q9BXY4

    Sequence Note

    Gln22 - His272

• Instruction

  • Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    Stability & Storage

    This product is stable after storage at:

    • -80°C for long-term storage under sterile conditions.

    Avoid repeated free-thaw cycles.

• Image

  Activity analysis of recombinant Human R-Spondin 3, His-SUMO Tag, CHO.
  R-Spondin 3 enhances BMP-2-mediated differentiation of MC3T3-E1 cells. The ED₅₀ for this effect is < 3 μg/mL.

  SDS-PAGE analysis of recombinant Human R-Spondin 3.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.