Human R-Spondin 1, His-SUMO Tag, CHO

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Catalog Number

LDG192PHM

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• Specifications

  • Species of Origin

    Human

    Expression system

    CHO

  • Affinity Tag

    His-SUMO Tag (N-term)

    Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

  • Purity

    >95% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 46.8 kDa.
    The protein migrates as 58-70 kDa under reducing condition (SDS-PAGE analysis).

  • Activity

    R-Spondin 1 enhances BMP-2-mediated differentiation of MC3T3-E1 cells. The ED₅₀ for this effect is < 3 μg/mL.

    Endotoxin level

    <0.1 EU per 1 μg of the protein by the LAL method.

  • Form

    Lyophilized

• Background

  • Background

    R-Spondin 1 (RSPO1) is a secreted growth factor that potentiates canonical Wnt/β-catenin signaling by binding to LGR4/5/6 receptors and neutralizing the activity of the E3 ubiquitin ligases RNF43 and ZNRF3. Through amplification of Wnt signaling, RSPO1 plays essential roles in stem cell maintenance, epithelial cell proliferation, tissue regeneration, and developmental processes. RSPO1 is widely used in cell biology and organoid research, particularly for the culture and expansion of intestinal and epithelial stem cells, and is also implicated in cancer-related Wnt signaling dysregulation.

    Synonyms

    RSPO1, Roof plate-specific spondin-1, Wnt signaling enhancer, LGR ligand RSPO1, Wnt agonist RSPO1, Stem cell growth factor

  • Uniprot ID

    Q2MKA7

    Sequence Note

    Ser21-Ala263

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  Activity analysis of recombinant Human R-Spondin 1, His-SUMO Tag, CHO.
  R-Spondin 1 enhances BMP-2-mediated differentiation of MC3T3-E1 cells. The ED₅₀ for this effect is < 3 μg/mL.

  SDS-PAGE analysis of recombinant Human R-Spondin 1 protein.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.