Leadgene Leadgene
Human Myoglobin, His Tag, E. coli
Catalog Number

LDG192PHE

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  • Specifications
    • Species of Origin

      Human

      Expression system

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Buffer

      Lyophilized from a 0.2 µm filtered solution of 50 mM Tris, 150 mM NaCl, pH 8.0

    • Purity

      >95% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 17.99 kDa.
      The protein migrates as 16-20 kDa under reducing condition (SDS-PAGE analysis).

    • Endotoxin level

      <0.1 EU per 1 μg of the protein by the LAL method.

      Form

      Lyophilized

  • Background
    • Background

      Myoglobin (MB) is a small, monomeric heme protein primarily found in heart and skeletal muscle tissue. It functions as an intracellular oxygen storage protein, capable of reversibly binding and releasing molecular oxygen. Myoglobin facilitates oxygen diffusion to mitochondria, especially during periods of high metabolic demand or hypoxia. Elevated levels in serum can indicate muscle injury, particularly myocardial infarction. It serves as a biomarker for muscle injury and is commonly used in cardiovascular research and diagnostic assay development.

      Synonyms

      Mb, PVALB-like protein, cardiac myoglobin, Myo

    • Uniprot ID

      P02144

      Sequence Note

      Met1-Gly154

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant human myoglobin

    SDS-PAGE analysis of recombinant human myoglobin

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  • Datasheet & Documents
    MSDS
    Manual
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