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Species of Origin
Human
Expression system
Escherichia coli
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Affinity Tag
His Tag (C-term)
Buffer
Lyophilized from a 0.2 µm filtered solution of 50 mM Tris, 150 mM NaCl, pH 8.0
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Purity
>95% as determined by SDS-PAGE analysis.
Molecular weight
The protein has a calculated MW of 17.99 kDa.
The protein migrates as 16-20 kDa under reducing condition (SDS-PAGE analysis). -
Endotoxin level
<0.1 EU per 1 μg of the protein by the LAL method.
Form
Lyophilized
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Specifications
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Background
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Background
Myoglobin (MB) is a small, monomeric heme protein primarily found in heart and skeletal muscle tissue. It functions as an intracellular oxygen storage protein, capable of reversibly binding and releasing molecular oxygen. Myoglobin facilitates oxygen diffusion to mitochondria, especially during periods of high metabolic demand or hypoxia. Elevated levels in serum can indicate muscle injury, particularly myocardial infarction. It serves as a biomarker for muscle injury and is commonly used in cardiovascular research and diagnostic assay development.
Synonyms
Mb, PVALB-like protein, cardiac myoglobin, Myo
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Uniprot ID
P02144
Sequence Note
Met1-Gly154
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Instruction
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Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
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Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
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Publication
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Datasheet & Documents
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Disclaimer:For Research Use or Further Manufacturing Only.