Species of Origin
His Tag (C-term)
Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
>98% as determined by SDS-PAGE analysis.
The protein has a calculated MW of 13.34 kDa.
The protein migrates as 13-26 kDa under reducing condition (SDS-PAGE analysis).
Measure by its ability to induce proliferation in NFS-60 cells. The ED₅₀ for this effect is <1 ng/mL. The specific activity of recombinant human M-CSF is approximately >2.5x 10⁸ IU/mg.
<0.1 EU per 1 μg of the protein by the LAL method.
Macrophage Colony Stimulating Factor (M-CSF) is a 18.54 kDa member of hematopoietic Growth Factors with 159 amino acid residues. M-CSF produced by osteoblasts and osteoblast precursors. M-CSF stimulates the growth and differentiation of the monocyte lineage, and promotes the survival, proliferation, and functions of mature monocytes/macrophages.
Processed macrophage colony-stimulating factor 1, Lanimostim, Macrophage colony-stimulating factor 1
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
Human M-CSF, His Tag, E. coli (LDG093PHE) induced NFS-60 cell proliferation, with the ED50 at 0.75 ng/mL.
SDS-PAGE analysis of recombinant human M-CSF.1/2
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Datasheet & Documents1/1
Disclaimer：For Research Use Only. Not for use in diagnostic or therapeutic procedures.