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Species of Origin
Human
Expression system
Escherichia coli
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Affinity Tag
His Tag (C-term)
Buffer
Lyophilized from a 0.2 µm filtered solution of 20 mM Acetate, pH 4.5.
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Purity
>95% as determined by SDS-PAGE analysis.
Molecular weight
The protein has a calculated MW of 9.9 kDa.
The protein migrates as 9 kDa under reducing condition (SDS-PAGE analysis). -
Activity
Measure by its ability to induce MCF-7 cells proliferation in a serum-free condition. The ED50 for this effect is <2.5 ng/mL.
Endotoxin level
<0.1 EU per 1 μg of the protein by the LAL method.
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Form
Lyophilized
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Specifications
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Background
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Background
LR3 IGF-1 (Long R3 Insulin-like Growth Factor-I) is a synthetic analog of IGF-1 engineered for increased potency and reduced binding to IGF-binding proteins. It stimulates cell proliferation and survival, and is widely used in cell culture and tissue engineering for enhanced growth effects.
Synonyms
Long-[Arg3]-Insulin-like Growth Factor 1, IGF-1 LR3, Long R3 IGF-I, IGF-I LR3, LR3 IGF-I
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Uniprot ID
P05019
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Instruction
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Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
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Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
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Image
![Activity analysis of recombinant Human LR3 IGF-1, His Tag, E. coli. Measure by its ability to induce MCF-7 cells proliferation in a serum-free condition. The ED50 for this effect is <2.5 ng/mL.]()
Activity analysis of recombinant Human LR3 IGF-1, His Tag, E. coli.
Measure by its ability to induce MCF-7 cells proliferation in a serum-free condition. The ED50 for this effect is <2.5 ng/mL.![SDS-PAGE analysis of recombinant Human LR3 IGF-1 protein.]()
SDS-PAGE analysis of recombinant Human LR3 IGF-1 protein.
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Publication
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Datasheet & Documents
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Disclaimer：For Research Use or Further Manufacturing Only.
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