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Leadgene Leadgene
Human Irisin, Tag Free, E. coli
Catalog Number

LDG176PHE

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  • Specifications
    • Species of Origin

      Human

      Expression system

      Escherichia coli

    • Affinity Tag

      Tag Free

      Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

    • Purity

      >90% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 12.70 kDa.
      The protein migrates as 10-17 kDa under reducing condition (SDS-PAGE analysis).

    • Endotoxin level

      <0.1 EU per 1 μg of the protein by the LAL method.

      Form

      Lyophilized

  • Background
    • Background

      Irisin is a transmembrane glycoprotein which is encoded by the Fibronectin type III domain-containing protein 5 gene. Irisin is secreted from skeletal muscle after exercise. Irisin induces the expression of thermogenin in adipocytes which prevents weight gain. The production of irisin is during the muscular contraction. Irisin likes a myokine after exercise which could do the conversion of white fat to brown fat in human.

      Synonyms

      Fibronectin type III domain-containing protein 5, Fibronectin type III repeat-containing protein 2

    • Uniprot ID

      #Q8NAU1

      Sequence Note

      Asp32-Glu143

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant human Irisin.

    SDS-PAGE analysis of recombinant human Irisin.

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  • Datasheet & Documents
    MSDS
    Manual
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