Leadgene Leadgene
Human IL-6, His Tag, CHO
Catalog Number

LDG069PHM

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  • Specifications
    • Species of Origin

      Human

      Expression system

      CHO

    • Affinity Tag

      His Tag (N-term)

      Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4

    • Purity

      >95% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 21.62 kDa.
      The protein migrates as 20-23 kDa under reducing condition (SDS-PAGE analysis).

    • Endotoxin level

      <0.1 EU per 1 μg of the protein by the LAL method.

      Form

      Lyophilized

  • Background
    • Background

      Human Interleukin-6 (IL-6) is a multifunctional cytokine that plays a vital role in immune response, inflammation, and hematopoiesis. It is involved in the acute phase response and is a key player in the body's defense against infections, as well as in chronic inflammation and autoimmunity. Elevated levels of IL-6 are often associated with inflammatory diseases and certain cancers. The Human IL-6 protein, when expressed in CHO (Chinese Hamster Ovary) cells, is typically tagged with a His (histidine) tag to facilitate its purification and detection in laboratory research. This recombinant IL-6-His fusion protein is extensively used in studies investigating inflammatory pathways, immune regulation, and the development of therapeutic interventions for diseases associated with IL-6 dysregulation.

      Synonyms

      Interleukin-6, IL-6, B-cell stimulatory factor 2, BSF-2, CTL differentiation factor, CDF, Hybridoma growth factor, Interferon beta-2, IFN-beta-2

    • Uniprot ID

      P05231

      Sequence Note

      Val30-Met212

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant Human IL-6.

    SDS-PAGE analysis of recombinant Human IL-6.

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  • Datasheet & Documents
    MSDS
    Manual
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