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Leadgene Leadgene
Human IL-6 ELISA Kit
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Catalog Number

LDG00025E

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  • Overview

    Components

    Interleukin-6 (IL-6) ELISA plate Stripwell microplate

    96 wells (12 x 8-well strips)

    Standard

    2 vials (Lyophilized form)

    Standard reconstitution buffer

    2 vials (1.1 mL)

    Standard & Sample diluent buffer

    1 vial (12 mL)

    Assay buffer

    1 vial (12 mL)

    HRP-antibody conjugate

    1 vial (70 μL)

    HRP-antibody conjugated diluent buffer

    1 vial (12 mL)

    20 X wash buffer

    1 vial (15 mL)

    TMB

    1 vial (12 mL)

    Stop solution

    1 vial (6 mL)

    Microplate sealing film

    1 sheet

    • Standard

      96 wells (12 x 8-well strips)

      2 vials (Lyophilized form)

    • Standard reconstitution buffer

      96 wells (12 x 8-well strips)

      2 vials (1.1 mL)

    • Standard & Sample diluent buffer

      96 wells (12 x 8-well strips)

      1 vial (12 mL)

    • Assay buffer

      96 wells (12 x 8-well strips)

      1 vial (12 mL)

    • HRP-antibody conjugate

      96 wells (12 x 8-well strips)

      1 vial (70 μL)

    • HRP-antibody conjugated diluent buffer

      96 wells (12 x 8-well strips)

      1 vial (12 mL)

    • 20 X wash buffer

      96 wells (12 x 8-well strips)

      1 vial (15 mL)

    • TMB

      96 wells (12 x 8-well strips)

      1 vial (12 mL)

    • Stop solution

      96 wells (12 x 8-well strips)

      1 vial (6 mL)

    • Microplate sealing film

      96 wells (12 x 8-well strips)

      1 sheet

  • Specifications
    • Reactivity

      Human

      Application

      Sandwich ELISA analysis

    • Sensitivity

      0.0042 ng/mL

      Specificity

      Human NF-L

    • Assay Range

      0.015625 ng/mL-1 ng/mL

      Sample Type

      Human Serum / Human Plasma

    • Recovery

      Serum
      Stock: 73.4%
      2x dilution: 94.4%
      4x dilution: 103.6%
      8x dilution: 104.4%

      Plasma
      Stock: 82.6%
      2x dilution: 84%
      4x dilution: 90.2%
      8x dilution: 93.6%

  • Background
    • Background

      Interleukin-6 (IL-6) is a pleiotropic, 22-28 kDa cytokine which plays fundamental role in the acute phase response, inflammation, bone metabolism, lymphocyte differentiation and cancer progression. Deregulation of IL-6 production was also found in several diseases, including rheumatoid arthritis, Alzheimer’s disease, autoimmune deficiency disease and different types of cancer.

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at 2-8°C for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • 2-8℃ for unopened product.

      Please refer to product manual for storage constructions.

  • Image
    Typical data The following data are for demonstration only.

    Typical data
    The following data are for demonstration only.

    An example of orientation of standards, blanks and samples in the stripwells microplate

    An example of orientation of standards, blanks and samples in the stripwells microplate

    Reagents provided and reconstitution

    Reagents provided and reconstitution

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  • Datasheet & Documents

Disclaimer:For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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