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Species of Origin
Human
Expression system
HEK293
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Affinity Tag
His Tag (C-term)
Buffer
Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
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Purity
>95% as determined by SDS-PAGE analysis.
Molecular weight
The protein has a calculated MW of 24.56 kDa.
The protein migrates as 35-50 kDa under reducing condition (SDS-PAGE analysis). -
Activity
Measured by its ability to bind human IL-4 in functional ELISA. Immobilized Recombinant human IL-4 at 1 μg/mL (100 μL/well) can bind Recombinant Human IL-4R Alpha Protein.
Endotoxin level
<0.1 EU per 1 μg of the protein by the LAL method.
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Form
Lyophilized
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Specifications
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Background
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Background
Interleukin-4 Receptor Alpha (IL-4Rα) is a receptor subunit that binds IL-4 and IL-13, mediating immune responses, particularly in allergic inflammation and asthma. It is crucial in the regulation of Th2 cell differentiation.
Synonyms
Interleukin-4 receptor subunit alpha, IL-4 receptor subunit alpha, IL-4R subunit alpha, IL-4R-alpha, IL-4RA, CD124, Soluble interleukin-4 receptor subunit alpha, Soluble IL-4 receptor subunit alpha, Soluble IL-4R-alpha, sIL4Ralpha/prot, IL-4-binding protein, IL4-BP
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Uniprot ID
P24394
Sequence Note
Met26 - His232
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Instruction
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Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
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Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
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Publication
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Datasheet & Documents
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Disclaimer:For Research Use or Further Manufacturing Only.