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Species of Origin
Human
Expression system
Escherichia coli
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Affinity Tag
His Tag (C-term)
Buffer
Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.
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Purity
>98% as determined by SDS-PAGE analysis.
Molecular weight
The protein has a calculated MW of 15.9 kDa.
The protein migrates as 12 kDa under reducing condition (SDS-PAGE analysis). -
Activity
Measure by its ability to induce TF-1 cells proliferation. The ED₅₀ for this effect is < 0.2 ng/mL. The specific activity of recombinant human IL-4 is approximately > 1 x 10⁷ IU/mg.
Endotoxin level
<0.1 EU per 1 μg of the protein by the LAL method.
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Form
Lyophilized
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Specifications
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Background
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Background
Interleukin-4 (IL-4) is a key cytokine produced by activated T cells, mast cells, basophils, neutrophils and eosinophils. IL-4 is critical for the development of Th2-mediated responses, which is related to allergy and asthma. It can also regulate B cell responses, including survival, cell proliferation and gene expression. IL-4 also plays fundamental role for B-cell stimulation, including induction of the IgE isotype switch.
Synonyms
Interleukin-4, B-cell stimulatory factor 1 , BSF-1, Binetrakin, Lymphocyte stimulatory factor 1, Pitrakinra
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Uniprot ID
#P05112
Sequence Note
His25-Ser153
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Instruction
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Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
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Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 2 weeks under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
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Review
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Publication
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Datasheet & Documents
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Disclaimer:For Research Use or Further Manufacturing Only.