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Catalog Number

LDG116PHM

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Specifications
- Species of Origin
  
  Human
  
  Expression system
  
  CHO
- Affinity Tag
  
  His Tag (C-term)
  
  Buffer
  
  Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
- Purity
  
  >95% as determined by SDS-PAGE analysis.
  
  Molecular weight
  
  The protein has a calculated MW of 22.5 kDa.
  The protein migrates as 30-38 kDa under reducing condition (SDS-PAGE analysis).
- Activity
  
  Measured by its ability to inhibit the IL-2-dependent proliferation of CTLL-2 cells. The ED50 for this effect is <1 µg/mL in the presence of 30 ng/mL of recombinant human IL-2.
  
  Endotoxin level
  
  <0.1 EU per 1 μg of the protein by the LAL method.
- Form
  
  Lyophilized
Background
- Background
  
  Interleukin-2 receptor alpha (IL-2RA, CD25) is a key component of the IL-2 receptor complex, essential for T-cell activation and immune regulation. It is a marker of Treg cells and immune disorders.
  
  Synonyms
  
  Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55, CD25
- Uniprot ID
  
  P01589
  
  Sequence Note
  
  Glu 22-Cys213
Instruction
- Reconstitution
  
  It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
  
  Shipping
  
  The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
- Stability & Storage
  
  This product is stable after storage at:
  - -20°C for 12 months in lyophilized state from date of receipt.
  - -20°C or -80°C for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
Image

HPLC analysis of recombinant Human IL-2RA, His Tag, CHO.
The purity of Human IL-2RA Protein is greater than 95% as determined by RP-HPLC.

Activity analysis of recombinant Human IL‐2RA, His Tag, CHO.
Measured by its ability to inhibit the IL-2-dependent proliferation of CTLL-2 cells. The ED50 for this effect is <1 µg/mL in the presence of 30 ng/mL of recombinant human IL-2.

SDS-PAGE analysis of recombinant Human IL‐2RA.

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Datasheet & Documents

Datasheet

MSDS

Manual

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