Human IGF-I, His Tag, E. coli

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Catalog Number

LDG088PHE

Datasheet

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• Specifications

  • Species of Origin

    Human

    Expression system

    Escherichia coli

  • Affinity Tag

    His Tag (C-term)

    Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

  • Purity

    >95% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 8.59 kDa.
    The protein migrates as 8-10 kDa under reducing condition (SDS-PAGE analysis).

  • Activity

    Measure by its ability to induce MCF-7 cells proliferation. The ED₅₀ for this effect is 0.9-3.1 ng/mL. The specific activity of recombinant human IGF-I is approximately >1.2 x 10³ IU/mg.

    Endotoxin level

    <0.01 EU per 1 µg of the protein by the LAL method.

  • Form

    Lyophilized

• Background

  • Background

    Insulin like Growth Factors 1 (IGF-I) is a 7.79 kDa member of the Insulin-like Growth Factors with 71 amino acid residues. IGF-I is mainly expressed from liver, adipose tissue, Endometrial stromal cells, Leydig cells, and can be isolated from plasma. IGF-I mediating the protein anabolic and promoting effect of pituitary growth hormone. IGF-I also affects metabolism of glycogen, DNA synthesis and glucose uptake via binding to IGF-I receptor.

    Synonyms

    Mechano Growth Factors, MGF, Somatomedin-C, Insulin-like Growth Factors

  • Uniprot ID

    #P05019

    Sequence Note

    Gly49-Ala118

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  Human IGF-I, His Tag, E. coli (LDG088PHE) induced MCF-7 cell proliferation, with the ED50 at 0.9084 ng/mL.

  SDS-PAGE analysis of recombinant human IGF-I.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.