Species of Origin
His Tag (C-term)
Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
>95% as determined by SDS-PAGE analysis.
The protein has a calculated MW of 8.59 kDa.
The protein migrates as 11 kDa under reducing condition (SDS-PAGE analysis).
Measure by its ability to induce MCF-7 cells proliferation. The ED₅₀ for this effect is 0.9-3.1 ng/mL. The specific activity of recombinant human IGF-I is approximately >1.2 x 10³ IU/mg.
<0.01 EU per 1 µg of the protein by the LAL method.
Insulin like Growth Factors 1 (IGF-I) is a 7.79 kDa member of the Insulin-like Growth Factors with 71 amino acid residues. IGF-I is mainly expressed from liver, adipose tissue, Endometrial stromal cells, Leydig cells, and can be isolated from plasma. IGF-I mediating the protein anabolic and promoting effect of pituitary growth hormone. IGF-I also affects metabolism of glycogen, DNA synthesis and glucose uptake via binding to IGF-I receptor.
Mechano Growth Factors, MGF, Somatomedin-C, Insulin-like Growth Factors
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
Human IGF-I, His Tag, E. coli (LDG088PHE) induced MCF-7 cell proliferation, with the ED50 at 0.9084 ng/mL.
SDS-PAGE analysis of recombinant human IGF-I.1/2
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Datasheet & Documents1/1
Disclaimer：For Research Use Only. Not for use in diagnostic or therapeutic procedures.