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Species of Origin
Human
Expression system
HEK293
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Affinity Tag
His Tag (C-term)
Buffer
Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
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Molecular weight
The protein has a calculated MW of 17.56 kDa.
The protein migrates as 24 kDa under reducing condition (SDS-PAGE analysis).Activity
Measure by its ability to induce cytotoxicity in HT29 cells, with the ED50 being < 1 ng/mL.
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Form
Lyophilized
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Specifications
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Background
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Background
Human Interferon Gamma (IFN-γ) is a cytokine that plays a crucial role in immune responses, particularly in the activation of macrophages and the promotion of Th1-type immune responses. It is a key player in the defense against viral infections, intracellular bacteria, and tumor cells. The Human IFN-γ protein, when expressed in HEK293 (Human Embryonic Kidney 293) cells, can be tagged with a His (histidine) tag, which simplifies its purification and detection for experimental purposes. This recombinant IFN-γ-His fusion protein is extensively used in research focusing on immunology, infectious diseases, cancer immunotherapy, and the study of cytokine signaling pathways.
Synonyms
Interferon gamma, IFN-gamma, Immune interferon
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Uniprot ID
GenBank: AAP20098.1
Sequence Note
Gln24-Gln166
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Instruction
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Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
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Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
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Image
![Activity analysis of recombinant Human IFN Gamma, His Tag, HEK293. Measure by its ability to induce cytotoxicity in HT29 cells, with the ED50 being < 1 ng/mL.]()
Activity analysis of recombinant Human IFN Gamma, His Tag, HEK293.
Measure by its ability to induce cytotoxicity in HT29 cells, with the ED50 being < 1 ng/mL.![SDS-PAGE analysis of recombinant Human IFN Gamma.]()
SDS-PAGE analysis of recombinant Human IFN Gamma.
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Publication
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Datasheet & Documents
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Disclaimer：For Research Use or Further Manufacturing Only.
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